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Novel tumor tissue preserving fluid and application thereof

A technology of tumor tissue and preservation solution, applied in the biological field, can solve problems such as inconsistency and affecting the growth of tumor tissue, and achieve the effect of improving the success rate

Inactive Publication Date: 2018-10-19
杭州安恺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the existing methods to improve the activity of tumor tissue in vitro are to add fetal bovine serum to the buffer or basal medium, but bovine serum is inconsistent with human tumor tissue and mouse receptor, and this difference may affect Growth of Tumor Tissue Transplanted into Mice

Method used

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  • Novel tumor tissue preserving fluid and application thereof
  • Novel tumor tissue preserving fluid and application thereof
  • Novel tumor tissue preserving fluid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A new type of tumor tissue preservation solution, including RPMI1640 medium 70%, human serum 15%, mouse serum 15%.

[0020] The mouse serum is obtained from mouse eyeballs or hearts. The human serum is AB type serum.

preparation Embodiment 1

[0022] A method for preparing the novel tumor tissue preservation solution described in Example 1, comprising the steps of:

[0023] (1) Take blood from the mouse heart or eyeball and place it in a centrifuge tube; place the centrifuge tube at 4°C for 1-5 hours; preferably place the centrifuge tube at 4°C for 2 hours. After balancing, perform centrifugation, and take the supernatant; continue to centrifuge the supernatant, and take the supernatant again. Store at -25°C;

[0024] The speed of centrifugation in step (1) can be set according to needs. However, it is best to centrifuge at a speed of 3000rpm for 10min after the balance, and take the supernatant; continue to centrifuge the supernatant at a speed of 3000rpm for 10min, and take the supernatant again Store at -15°C - 25°C.

[0025] (2) Draw 70 parts of RPMI1640 medium into the centrifuge tube with a pipette gun;

[0026] (3) Then use a pipette gun to draw 15 parts of human serum and 15 parts of mouse serum into a ce...

Embodiment 3

[0029] The application of a novel tumor tissue preservation solution prepared in Example 1 comprises the following steps:

[0030] The operating environment for tumor tissue sampling needs to be clean or above to prevent tissue contamination. The best place to take tumor tissue is where the tumor is actively growing, in good condition, and where there is less connective tissue. Obtain specimens with a size of at least 0.5cm*0.5cm*0.5cm. The removed specimen must be immediately put into a sterile tube containing a specific medium and stored in a 4°C cold-chain transport box. Pay attention to the placement method to ensure that it does not freeze.

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Abstract

The invention relates to biotechnology and particularly discloses a novel tumor tissue preserving fluid and application thereof. The novel tumor tissue preserving fluid is prepared from a culture medium RPMI1640, human serum and mouse serum. The novel tumor tissue preserving fluid can keep the biological activity of the living tumor tissue taken out from the human body so as to improve the successrate of transplanting the tumor tissue into the mouse body.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel tumor tissue preservation solution and its application. Background technique [0002] Human-derived tumor xenograft (Patient-Derived Xenograft, PDX) model is a tumor model grown by directly transplanting fresh tumor tissues (puncture samples or surgical samples) from patients into immunodeficient mice. Compared with the traditional cell line model, the PDX model can better reflect the biological characteristics of the patient's tumor. The PDX model not only retains the similar proliferation and histopathological characteristics of the patient's tumor tissue, but also in terms of biological behavior, including tumor genes, Molecular features such as proteins are highly consistent with patient tumor tissue. [0003] The construction of PDX models requires fresh human tumor tissue samples. Therefore, in order to ensure the success rate of transplantation, during the tumor tissu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 滕斐
Owner 杭州安恺生物科技有限公司
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