Detection method combined with rotating ultra-thin layer chromatography and surface enhanced Raman scattering technology
A surface-enhanced Raman and detection method technology, applied in the detection field, can solve the problems of non-reusable characteristics, high detection cost, waste of resources and labor, etc., and achieve the effect of low cost, high sensitivity and simple operation process
Inactive Publication Date: 2018-10-19
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology
[0003] However, surface-enhanced Raman scattering substrates usually need to be formed through complex physical or chemical methods, and most of them do not have the characteristics of reusability, so there is a waste of resources and labor, and the problem of increased detection costs
Method used
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Embodiment 1
[0018] Example 1: TLC-Surface Enhanced Raman Spectroscopy combined use of six synthetic pigments.
[0019] The specific operation steps are as follows.
[0020] 1-1. Fix a glass substrate with a length of 5 cm and a width of 1 cm on the surface of the sample stage with heat-resistant Teflon tape, facing the direction of steam incidence, when the chamber pressure is less than 10 -6 During Torr, a layer of 20nm titanium film is evaporated by electron beam deposition coating method to make the surface structure have good adhesion with the glass substrate. Raman scattering structure, after the cavity is cooled, make the normal line of the sample stage and the steam incident direction be 85°, continue to evaporate 2000nm silver film to form the upper surface enhanced Raman scattering surface enhanced Raman scattering structure, and finally obtain the surface enhanced Raman scattering structure. Mann scattering basis.
[0021] 1-2. Add 2 μL of the sample to be tested dropwise on b...
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The invention discloses a detection method combined with rotating ultra-thin layer chromatography and surface enhanced Raman scattering technology. The method comprises the steps of (A) preparing a surface-enhanced Raman scattering substrate: depositing a titanium film and a silver film on the surface of a glass substrate by using the tilt angle deposition technology, and continuing to deposit thesilver film after adjusting a deposition angle, (B) carrying out rotating thin layer chromatography expansion: carrying out rotating thin layer chromatography development on a sample on the surface-enhanced Raman scattering substrate by using a methanol as a developing agent, and (C) carrying out surface enhancement Raman spectrum detection: carrying out Raman spectrum detection on the developedsample. Thereby, different samples to be detected can be quickly distinguished, and the method has the advantages of a simple operation process, low cost and high sensitivity.
Description
technical field [0001] The present invention relates to a detection method for combined use of rotating ultra-thin layer chromatography and surface-enhanced Raman scattering technology, in particular, to a substrate preparation and The use method belongs to the technical field of detection. Background technique [0002] At present, surface-enhanced Raman spectroscopy has become a powerful analytical tool, which extends the surface vibrational spectrum and provides possible solutions to a large number of chemical problems. In reagent diagnosis, some ideal surface-enhanced Raman scattering substrates have high requirements, such as: high enhancement performance, good repeatability, strong uniformity, stable life, and simple manufacture. [0003] However, surface-enhanced Raman scattering substrates usually need to be formed through complex physical or chemical methods, and most of them do not have the characteristics of reusability, so there is a waste of resources and labor,...
Claims
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Login to View More IPC IPC(8): G01J3/44
CPCG01J3/44
Inventor 韩彩芹姚悦
Owner XUZHOU NORMAL UNIVERSITY