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Long noncoding RNA SNHG15 and application thereof in preparation of cancer diagnosis and treatment drugs

A non-coding, cancer technology, applied in the field of genetic engineering, can solve problems such as unclear function of long non-coding RNA

Inactive Publication Date: 2018-10-26
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the functions of many abnormally expressed long non-coding RNAs in the process of tumor EMT are not clear, and further research is needed to reveal their functions in tumors

Method used

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  • Long noncoding RNA SNHG15 and application thereof in preparation of cancer diagnosis and treatment drugs
  • Long noncoding RNA SNHG15 and application thereof in preparation of cancer diagnosis and treatment drugs
  • Long noncoding RNA SNHG15 and application thereof in preparation of cancer diagnosis and treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 Searching for differentially expressed long non-coding RNAs through data mining

[0104] Previous studies have shown that long non-coding RNAs can be involved in a variety of biological processes, including the development of organisms and tumor progression. In order to explore the role of long non-coding RNA in the development of human colorectal cancer and its molecular mechanism, we decided to conduct data mining and analysis on the RNA-seq data in the existing public database. We found and downloaded the RNA-seq dataset numbered GSE50760 in the public database GEO (Gene Expression Omnibus). The data set is derived from the tissue RNA sequencing results of 18 patients. Each patient contained three different tissue types, including normal intestinal epithelial tissue (more than 5 cm from the tumor margin) (NC), colorectal carcinoma in situ (PC) and colorectal cancer metastases in the liver ( MC), containing a total of 54 RNA-seq data. The expression level...

Embodiment 2

[0105] Example 2 High expression of long non-coding RNA SNHG15 is associated with poor prognosis of tumors

[0106] To examine the expression of long non-coding RNA SNHG15 in tumors, information from TCGA and UCSC CancerGenomics databases was used. We downloaded the following data from Cancer Browser (https: / / genome-cancer.ucsc.edu / ): Pancan normalized (Gene expression was produced via RNA-seq and mean-normalized across all TCGA cohorts) gene expression dataset, including Colorectal cancer (Colonadenocarcinoma, COAD, sample size = 329), breast cancer (Invasive breast carcinoma, BRAC, sample size = 1215) and renal clear cell carcinoma (Kidney clear cell carcinoma, KIRC, sample size = 606). We also downloaded the DNA methylation chip data (Methylation450k), which contains: colorectal cancer (COAD, sample size = 329), breast cancer (BRCA, sample size = 872) and renal clear cell carcinoma (KIRC, sample size =480). Sample comparisons were analyzed using the Student’s t test. Ove...

Embodiment 3

[0107] Example 3 Long non-coding RNA SNHG15 promotes tumor cell metastasis

[0108] To explore the role of long non-coding RNA SNHG15 in tumor progression, we performed experiments on cellular functions. We introduced the SNHG15 expression vector contained in the virus into the colorectal cancer cell lines HCT116, SW1116 and SW480 by virus infection. Subsequent functional tests showed that overexpression of long non-coding RNA SNHG15 could promote the metastatic ability of colorectal cancer cells ( image 3 A-C).

[0109] According to the above experiments, we speculate that the long non-coding RNA SNHG15 may be involved in the process of tumor invasion and metastasis. Therefore, we detected the expression of molecules related to tumor EMT in HCT116, SW1116 and SW480 stable cell lines by Western blot method. The experimental results showed that overexpression of long non-coding RNA SNHG15 could also up-regulate the expression of EMT-related transcription factor Slug in HCT1...

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Abstract

The invention belongs to the technical field of genetic engineering, and discloses long noncoding RNA SNHG15 and application thereof in preparation of cancer diagnosis and treatment drugs. Cancers include colorectal cancer, breast cancer, kidney cancer and the like. Expression of the long noncoding RNA SNHG15 in tumor tissues of patients suffering from the colorectal cancer, the breast cancer andthe kidney cancer is up-regulated. The patients with highly-expressed long noncoding RNA SNHG15 have poor prognosis. Through expression knock-down of the long noncoding RNA SNHG15 in colorectal cancercell lines, invasion, migration and multiplication capabilities of cells can be inhibited, and drug susceptibility of colorectal cancer cell lines to antitumor drugs including gemcitabine and cis-platinum can be improved.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a long non-coding RNA SNHG15 and its application in the preparation of drugs for diagnosis and treatment of cancer. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in men and women worldwide, and its death rate ranks fourth among all cancer patients. More than 1.2 million people are diagnosed with colorectal cancer each year. Although the application of endoscopic technology has enabled patients with colorectal cancer to obtain early diagnosis opportunities, and new treatment methods for colorectal cancer have gradually been established, the research on the specific molecular mechanism behind the occurrence and development of colorectal cancer is still incomplete. The problem awaits further disclosure. Following proteins and small RNAs, long non-coding RNAs, which have been research hotspots in recent years, also play imp...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/106C12Q2600/118C12Q2600/178
Inventor 张宏权江浩战军
Owner PEKING UNIV
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