Method for detecting galantamine in lycoris aurea

A technology of galantamine and printed electrodes, which is applied in the field of nano-gold modified printed electrodes to detect galantamine, can solve the problems of low sensitivity and inability to directly detect galantamine, so as to improve sensitivity and shorten the time required the effect of time

Active Publication Date: 2018-10-30
上海联星医药科技有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited performance of the printed electrode itself, this method has the disadvantage of low sensitivity, so that it cannot be used for the direct detection of galantamine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for detecting galantamine in sudden laughter, the operation steps are as follows:

[0026] (1) Sample processing: After chopping the bulb of Hudixiao, use a clean food mixer to grind it until it becomes a paste, weigh 10g of the sample into a 50mL centrifuge tube, add 20mL of methanol, cover the tube cap, and put the cap on the tube Place it in an ultrasonic machine for ultrasonic extraction for 10 minutes, the ultrasonic power is 200W, and the ultrasonic frequency is 20kHz. The resulting dispersion was filtered through polyethersulfone filter paper with a pore size of 200 nm to obtain the extract of Hudixiao.

[0027] (2) Preparation of nano-gold modified printed electrode sensor: wash the surface of the printed electrode with distilled water and ethanol respectively, and place the cleaned printed electrode in a mixed solution of 20mL dilute hydrochloric acid (0.1M) and chloroauric acid (0.1wt%), Electrodeposition was performed at +0.4V for 1 min to prepare n...

Embodiment 2

[0031] A method for detecting galantamine in sudden laughter, the operation steps are as follows:

[0032] (1) Sample processing: After chopping the bulb of Hudixiao, use a clean food mixer to grind it until it becomes a paste, weigh 5g of the sample into a 50mL centrifuge tube, add 10mL of methanol, cover the tube cap, and put the cap on the tube Placed in an ultrasonic machine for ultrasonic extraction for 10 min, the resulting dispersion was filtered through polyethersulfone filter paper with a pore size of 2 μm to obtain the extract of Hudixiao.

[0033] (2) Preparation of nano-gold modified printed electrode sensor: wash the surface of the printed electrode with distilled water and ethanol respectively, and place the cleaned printed electrode in a mixed solution of 20mL dilute hydrochloric acid (0.2M) and chloroauric acid (0.5wt%), The nano-gold modified printed electrode sensor was prepared by constant potential electrodeposition at +0.5V for 5min.

[0034] (3) Drawing ...

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Abstract

The invention discloses a method for detecting galantamine in lycoris aurea, and relates to plant sample handling, preparation of a nanogold printing electrode sensor and electrochemical detection. According to the method, the nanogold-modified printing electrode sensor is adopted to fully absorb in a sample extracting solution, then the nanogold printing electrode sensor is subjected to differential pulse voltammetry scanning to measure current values at oxidation peaks, within a detectable range, the content of the galantamine in the lycoris aurea absorbed by the nanogold printing electrodesensor is in a linear relation with the current intensities at the oxidation peaks, and according to a working curve, the content of the galantamine in the detected lycoris aurea can be calculated. The method has the advantages that the nanotechnology and the silk-screen printing technology are combined by the prepared nanogold printing electrode sensor, sensitivity, in galantamine concentration detection, of the sensor can be improved remarkably, and the minimum limit of detection of the sensor can reach to 0.013 microns.

Description

technical field [0001] The invention relates to a method for detecting galantamine in herbaceous flowers of the genus Lycoris, in particular to a method for detecting galantamine by a nano-gold modified printed electrode. Background technique [0002] Hudixiao is a perennial herbaceous flower of the genus Amaryllis. It grows mostly in a cool and dark place. It is warm in nature, flat and sweet in taste, and has the effects of reducing swelling, relieving pain, dispelling toxins, and inducing vomiting. Galantamine is a secondary metabolite alkaloid of Hudixiao, which is mainly distributed in its bulbs. It is recorded in the "Chinese Pharmacopoeia" that the injections and tablets of galantamine hydrobromide can be used to treat nervous system disorders. Sensory or movement disorders caused by it, including the treatment of sequelae of poliomyelitis, myasthenia gravis, and intestinal paralysis, as well as anti-curare drugs and wake-up agents after surgical anesthesia, etc. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/26
CPCG01N1/28G01N27/26
Inventor 傅力
Owner 上海联星医药科技有限公司
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