A method for promoting the accumulation of astaxanthin in Haematococcus pluvialis
A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of promoting the accumulation of Haematococcus pluvialls astaxanthin, which can solve the problem of poor quality, insufficient light, delay of Haematococcus pluvialls astaxanthin, and restriction of astaxanthin in Haematococcus pluvialls. Production efficiency and other issues, to achieve the effect of increasing content and improving production efficiency
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Embodiment 1
[0019] At a light intensity of 150μmol / m 2 / s and the light-to-dark ratio of 14h / 10h cultured Haematococcus pluvialis cells reached the plateau stage, and the cell density was 508,900 cells / mL. Dilute the Haematococcus pluvialis cells to 142,200 cells / mL with tap water, add glycerol to a final concentration of 0.014mol / L, and 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin.
[0020] Depend on figure 1 It can be seen that from the 5th day onwards, compared with the control (no glycerol group), adding glycerol at one time significantly increased the content of astaxanthin in Haematococcus pluvialis, indicating that glycerol can promote Penicillin accumulation.
Embodiment 2
[0022] At a light intensity of 200μmol / m 2 / s and the light-to-dark ratio of 14h / 10h cultured Haematococcus pluvialis cells reached the plateau stage, and the cell density was 410,300 cells / mL. Add glycerol directly to the cells of Haematococcus pluvialis to a final concentration of 0.01mol / L, and in the light intensity of 2500μmol / m 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin.
[0023] Depend on figure 2 It can be seen that from the 5th day onwards, compared with the control group (without adding glycerol), the one-time addition of glycerin significantly increased the content of astaxanthin in Haematococcus pluvialis, indicating that glycerol can promote the growth of Haematococcus pluvialis shrimp under strong light Penicillin accumulation.
Embodiment 3
[0025] At a light intensity of 180μmol / m 2 / s and the light-to-dark ratio of 14h / 10h cultured Haematococcus pluvialis cells reached the plateau stage, and the cell density was 523,400 cells / mL. Dilute the Haematococcus pluvialis cells to 201,200 cells / mL with tap water, add glycerol to a final concentration of 0.02mol / L, and 2 / s and the light-dark ratio of 14h / 10h induced the accumulation of astaxanthin.
[0026] Depend on image 3 It can be seen that from the 5th day onwards, compared with the control group (without adding glycerol), the one-time addition of glycerin significantly increased the content of astaxanthin in Haematococcus pluvialis, indicating that glycerol can promote the growth of Haematococcus pluvialis shrimp under strong light Penicillin accumulation.
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