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Construction method for HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules

A construction method and feature map technology, applied in the field of medicine, can solve the problems of no relevant reports on the construction method, reaction product quality, control product quality, etc., and achieve the effects of scientific and reliable method, good reproducibility, and good separation effect.

Active Publication Date: 2018-11-02
吉林修正药业新药开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the patents related to quality control are only for the content determination and qualitative identification of an active ingredient of a blind drug, and it is difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole
At present, there is no relevant report on the construction method of the HPLC characteristic map of Jingqi Shuangshen Capsules

Method used

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  • Construction method for HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules
  • Construction method for HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules
  • Construction method for HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: A method for constructing the HPLC characteristic map of Jingqi Shuangshen capsules

[0056] Instruments: UltiMate 3000 high-performance liquid chromatograph, UV DAD detector; Mettler AE240 one hundred thousandth analytical balance (Shanghai Mettler-Toledo Instruments Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo) Instrument Co., Ltd.) etc.

[0057] Reagents: methanol is analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0058] Reagent: Ginsenoside Rg 1 , Salvianolic acid B, formononetin and tanshinone IIA (all purchased from China Institute for Food and Drug Control).

[0059] Methods and results

[0060] (1) Preparation of test solution: Take about 1g of Jingqi Shuangshen capsule content, accurately weigh it, accurately add 10mL methanol, weigh it, sonicate for 20 minutes, let it cool, and use methanol to make up the lost weight. Filter, take the additional filtrate, and get it;

[0061...

Embodiment 2

[0066] Example 2: A method for constructing the HPLC characteristic map of Jingqi Shuangshen capsules

[0067] Instruments: UltiMate 3000 high performance liquid chromatograph, UV DAD detector; Mettler AE240 one hundred thousandth analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.) etc.

[0068] Reagents: methanol is analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0069] Reagent: Ginsenoside Rg 1 , Salvianolic acid B, formononetin and tanshinone IIA (all purchased from China Institute for Food and Drug Control).

[0070] Methods and results

[0071] (1) Preparation of test solution: Take about 2g of Jingqi Shuangshen capsule content, accurately weigh it, accurately add 25mL methanol, weigh it, sonicate for 30 minutes, let it cool, and use methanol to make up the lost weight. Filter, take the additional filtrate, and get it;

[0072] ...

Embodiment 3

[0075] Example 3: A method for constructing the HPLC characteristic map of Jingqi Shuangshen capsules

[0076] Instruments: UltiMate 3000 high performance liquid chromatograph, UV DAD detector; Mettler AE240 one hundred thousandth analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.) etc.

[0077] Reagents: methanol is analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0078] Reagent: Ginsenoside Rg 1 , Salvianolic acid B, formononetin and tanshinone IIA (all purchased from China Institute for Food and Drug Control).

[0079] Methods and results

[0080] (1) Preparation of test solution: Take about 3g of Jingqi Shuangshen capsule content, accurately weigh it, accurately add 50mL methanol, weigh it, sonicate for 40 minutes, let it cool, and use methanol to make up the lost weight. Filter, take the additional filtrate, and get it;

[0081] ...

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Abstract

The invention relates to the technical field of medicines, in particular to a construction method for an HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules. The construction method comprises the following steps: taking the content of the fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules, adding methanol, filtering the mixture after ultrasonic treatment, and taking a continuous filtrate to obtain a solution of a sample for test; taking a ginsenoside Rg1 reference substance, a danshinolic acid B reference substance, a formononetin reference substance and a tanshinone IIA reference substance to be mixed, and adding methanol to obtain a reference substance solution; testing the solution of the sample for test and the reference substance solution by means of high performance liquid chromatography separately to obtain the HPLC specific chromatogram of the fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules, wherein the chromatographic condition of the high performance liquid chromatography is as follows: by taking a C18 column as a chromatographic column, acetonitrile as a moving phase A and a 0.1%phosphoric acid solution as a moving phase B, gradient elution is carried out. The specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules established by the method is relatively good in separating degree, and meanwhile, 15 active components of traditional Chinese medicines can be separated and detected at the same time.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing an HPLC characteristic map of Jingqi Shuangshen capsules. Background technique [0002] Jingqi Shuangshen Capsule is a traditional Chinese medicine compound composed of four flavors: astragalus, ginseng, danshen and Huangjing. Its main functions are tonifying qi and nourishing yin, promoting blood circulation and removing blood stasis, nourishing the heart and calming the mind. It is suitable for cardio-cerebrovascular diseases such as palpitations, shortness of breath, dizziness, headache, fatigue, tinnitus, vertigo, numbness of the limbs, loss of eyes and forgetfulness. The current implementation of the national drug standard number is WS-5136(B-0136)-2002. The standard only includes properties, inspections, and identification items and content determination items for individual medicinal materials. Due to the many flavors of traditional Chinese medicine c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴萍徐建白冰李亚东崔宪利
Owner 吉林修正药业新药开发有限公司
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