Regulation and control research method of cell cilium transportprotein 80 in rapid maxillary expansion mechanical biological signal transduction

Abstract

The invention discloses a regulation and control research method of cell cilium transportprotein 80 in rapid maxillary expansion mechanical biological signal transduction. The method comprises the following steps: a, establishing a rapid maxillary expansion mouse animal model; b, establishing IFT80 gene silence osteoblast; c, establishing an in-vitro stretching culture osteoblast model; d, establishing a co-culture model of osteoblast and osteoclast; e, carrying out in-vivo transfection experiment; f, confirming a relationship of IFT80 spatio-temporal expression and rapid maxillary expansion midline palatine suture bone remolding; g, confirming regulation and control mechanisms of IFT80 upon proliferation and differentiation of in-vitro stretching cultured osteoblast, primary cilium formation and mechanical biological signal transduction; h, confirming influence of IFT80 gene silence upon in-vitro stretching cultured osteoblast in regulating functions of osteoclast. The method has great instruction significances for treating such malocclusion.

Description

Technical field

[0001] The present invention relates to the technical field of biological genes, and in particular to a method for studying the regulation mechanism of the intraciliary transporter 80 in the upper jaw rapid expansion mechanical biological signal transduction. Background technique

[0002] Underdevelopment of the maxillary width is a common malocclusion in orthodontics. Rapid Maxillary Expansion (RME) is the most commonly used method to treat such malocclusion. The principle is to guide the upper jaw to move left and right by expanding the palatal suture, and then the expanded palatal suture tissue proliferates, repairs and deposits bone. The top of the upper jaw is widened, and the width of the upper dental arch is increased, finally achieving the purpose of treatment. However, after rapid expansion of the palatal suture, the mineralization and regeneration of new bone takes more time. The new bone that has not been calcified at the edge of the palatal suture can ...