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Method for determining plant origin small RNA in human body by detecting faeces

A plant-derived, plant-based technology, applied in the field of determining plant-derived small RNAs, can solve problems such as unreported plant miRNA detection technology

Inactive Publication Date: 2018-11-06
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the detection technology of related plant miRNAs from human body using feces samples

Method used

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  • Method for determining plant origin small RNA in human body by detecting faeces
  • Method for determining plant origin small RNA in human body by detecting faeces
  • Method for determining plant origin small RNA in human body by detecting faeces

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Embodiment Construction

[0026] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the drawings and embodiments. It should be declared that the specific examples described here are only used to explain the present invention, and are not intended to limit the scope of the present invention. In addition, in the following description, some descriptions of known technologies are omitted to avoid unnecessarily confusing the concept of the present invention.

[0027] The specific implementation examples are as follows:

[0028] Step 1. Sample collection:

[0029] Three parts of feces samples used in experiments in the present invention were donated by three individuals from volunteers, and the extraction steps were as follows:

[0030] Take samples from the collection box (bottle) or give the test card and sampling stick to the volunteers to take samples by themselves. When samp...

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Abstract

The invention relates to the technical field of gene detection, and aims to provides a method for determining plant origin small RNA in a human body by detecting faeces. The method comprises the following steps: collecting human faeces samples by adopting a conventional method; extracting total RNA of the faeces samples by using an RNA extraction kit; establishing a small RNA library according toconventional small RNA library development flow and method; performing high-throughput sequencing on the small RNA in the faeces samples by using a high-throughput sequencing technology platform; andperforming bioinformatics analysis of the small RNA data on the sequencing result to determine plant origin miRNA in the human body and miRNA abundance. Small RNA sequencing is performed on the humanfaeces samples, the type and the abundance of the plant miRNA in the samples can be identified by using a bioinformatics method, the faeces samples can be used for replacing blood to analyze the relation between the abundance of the plant miRNA in the human body and the diet, and the faeces samples are used as enteric microorganism detection supplementation, and more information is provided.

Description

technical field [0001] The invention belongs to the technical field of gene detection, in particular to a method for sequencing and analyzing small RNA samples in a human body and determining plant-derived small RNAs. Background technique [0002] Single-stranded small RNAs that do not code proteins are a new class of genes with regulatory functions discovered in the past 10 years, and microRNA (miRNA) is one of the important small RNAs. The length of miRNA is 20-24 nucleotides, which can bind to target gene mRNA through sequence matching, especially the 3'-untranslational region (3'-UTR) of mRNA. If the sequence is an exact match, this will result in cleavage of the target mRNA, and the fragmented mRNA will then be degraded. If the miRNA and the target mRNA are not perfectly complementary, this will inhibit the translation of the mRNA into protein without affecting the stability of the mRNA. [0003] In recent years, plant-derived miRNAs have been found on a large scale i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869G06F19/22
CPCC12Q1/6869C12Q2535/122
Inventor 樊龙江孙硕
Owner ZHEJIANG UNIV
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