Method for raising green seedling differentiation ratio cultivated by wheat anther

Abstract

A method for increasing the differentiation ratio of green wheat seedlings cultured with wheat pollen features that the extracted liquid from potato and mother liquid of iron salt are added to induct culture medium while the culture condition is modified to greatly increase the differentiation ratio and yield rate of green seedlings and decrease the albino rate. It creates a new approach for the cytogenetic research of wheat haploid or ampliploid population and the wheat-ploid culure research with lower energy consumption and cost.

Description

technical field [0001] The invention relates to the technical field of botany research, in particular to a method for improving the green seedling differentiation ratio of wheat anther culture. Background technique [0002] Wheat anther culture is a key technology for cultivating wheat haploids, creating wheat double haploid populations for cytogenetic research and wheat ploidy breeding. The current common wheat anther culture method is to use C 17 or W 14 The induction medium was cultured at about 32°C. Commonly used C 17 The main components and proportions of the induction medium are (mg / L): [0003] Macroelement compounds: KNO 3 1400 [0004] K H 2 PO 4 400 [0005] NH 4 NO 3 300 [0006] MgSO 4 ·7H 2 O 150 [0007] CaCl 2 2H 2 O 150 [0008] Trace element compound: MnSO 4 4H 2 O 11.2 [0009] ZnSO 4 ·7H...

AI Technical Summary

Problems solved by technology

[0047] 1. In the process of wheat anther culture with common induction medium, the healing rate, number of green seedlings, green seedling differentiation rate and green seedling yield are all low, and more than 80% of meaningless albino seedlings are differentiated

[0048] 2. The cultivation temperature is about 32°C, because the cultivation temperature is higher, and more energy is consumed