Enzyme-assisted extraction method of phospholipid shrimp oil in krill and detection method

Abstract

The invention discloses an enzyme-assisted extraction method of phospholipid shrimp oil in krill and a detection method, and the enzyme-assisted extraction method comprises the following steps: 1, preparing minced krill; 2, mixing the minced krill with a trypsin solution to obtain a mixture A; 3, stirring the mixture A at a constant temperature of 40-41.5 DEG C for 2.8 h to 3.2 h; 4, cooling to 18DEG C to 20 DEG C, and storing at a constant temperature for 20 min to 25 min; and 5, using Bligh Dyer method to extract the phospholipid shrimp oil in the krill. On one hand, more kinds of fatty acids can be extracted at a higher extraction rate by the enzyme-assisted extraction method, the content of unsaturated fatty acids is higher, and the nutritional quality of the phospholipids is better.On the other hand, the detection method can quickly and accurately detect the phospholipids in the phospholipid shrimp oil.

Description

technical field [0001] The invention relates to an enzyme-assisted extraction method and a detection method of krill (Antarctic krill) phospholipid shrimp oil, belonging to the technical field of food production. Background technique [0002] Antarctic krill is a crustacean that mainly lives in the waters of Antarctica and is rich in biological resources. Although its volume is small, it is rich in protein and phospholipids. Phospholipids contain a large amount of w-3 series polyunsaturated fatty acids, especially EPA (eicosapentaenoic acid) and DHA (eicosa hexaenoic acid). EPA can reduce the content of cholesterol and blood viscosity, promote blood circulation. DHA can prevent cardiovascular disease and cancer, strengthen the brain and improve eyesight. Compared with the lipids in traditional fish oils, the lipids in Antarctic krill have higher absorbability and no toxic side effects, so they have higher nutritional value. At present, the raw materials for producing phos...

AI Technical Summary

Problems solved by technology

Although the solvent extraction method has high separation efficiency and is convenient for continuous operation, the extraction rate of phospholipids is low, the operation steps are cumbersome, and the content of residual organic solvents in phospholipids often exceeds the standard, and some organic solvents have high toxicity. The use of the method is restricted

Supercritical extraction and ultrasonic enzyme-assisted extraction have the advantages of low operating temperature and difficult to destroy the extracted components, but the equipment of these methods is expensive and the production cost is high