Method and system for detecting meat and bone meal species on basis of infrared spectrum of genomic DNA

A technology of infrared spectroscopy and detection method, applied in the field of meat and bone meal species detection, can solve the problems affecting the accuracy of detection results, long detection time and high analysis cost, and achieve the effect of improving species detection efficiency and high purity

Active Publication Date: 2018-11-13
CHINA AGRI UNIV
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  • Description
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Problems solved by technology

However, this method is expensive to analyze, takes a long time to detect, and has strict requirements on the detection environment. Moreover,

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  • Method and system for detecting meat and bone meal species on basis of infrared spectrum of genomic DNA
  • Method and system for detecting meat and bone meal species on basis of infrared spectrum of genomic DNA
  • Method and system for detecting meat and bone meal species on basis of infrared spectrum of genomic DNA

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Effect test

Embodiment 1

[0093] Example 1 Meat and Bone Meal Species Detection Method Based on Genomic DNA Infrared Spectroscopy

[0094] 51 meat and bone meal feed products of known animal species were obtained as research samples, including 12 pork bone meal, 11 chicken bone meal, 14 beef bone meal and 14 mutton bone meal. In order to ensure the representativeness of meat and bone meal samples, all meat and bone meal raw materials were collected in batches from multiple markets in Beijing. In addition, in order to ensure the quality of meat and bone meal samples, all samples were self-made in the laboratory. According to EU regulations, all samples were cooked (133°C for 20 min), dried, extruded and degreased, and pulverized to 0.5mm. The sodium laurate extraction method was used to extract the genomic DNA samples. In order to overcome the difference caused by the solubility of the DNA solution, the genomic DNA solution (A260 / A280 between 1.8-2.0) in the extracted 51 meat and bone meal samples was...

Embodiment 2

[0133] The optimization of embodiment 2 meat and bone meal genome DNA extraction method

[0134] In order to obtain high-quality infrared spectrograms, the present invention adopts 5 methods to extract genomic DNA of meat and bone powder.

[0135] Method 1: According to the SOP standard method of the EU animal protein testing reference laboratory, the genomic DNA of the meat and bone meal samples was extracted using the Wizard Magnetic DNA Purification Kit (Promega, USA).

[0136] Method 2: On the basis of method 1, the sample was purified using an ultrafiltration tube (10K, Millipore, USA). Purification steps were performed according to the instructions.

[0137] Method 3: On the basis of method 1, the sample was purified by the magnetic bead method (FineMag DNA Clean Beads, Jifan Biotechnology, China), and the purification steps were performed according to the instructions.

[0138] Method 4: Use TIANamp Feedstuff Animal DNA Kit (Tiangen, China) and follow the instructions...

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Abstract

The invention provides a method and a system for detecting meat and bone meal species on the basis of an infrared spectrum of genomic DNA. The method comprises the following steps: 1) extracting genomic DNA of different species of meat and bone meal samples with known sources; 2) acquiring infrared spectral information of the DNA samples; 3) associating the acquired infrared spectral information with species source information of the DNA samples, and establishing a meat and bone meal species discriminant analysis model; 4) extracting the genomic DNA of a to-be-detected meat and bone meal sample with the method in step 1), acquiring the infrared spectral information of a to-be-detected DNA sample with the method in step 2), and substituting the information into the discriminant analysis model to obtain the species source information of the to-be-detected meat and bone meal sample. With adoption of the method and the system for detecting the meat and bone meal species on the basis of theinfrared spectrum of genomic DNA, detection precision and detection efficiency for the bone meal species are improved remarkably.

Description

technical field [0001] The invention relates to the technical field of feed detection, in particular to a method and system for detecting the species of meat and bone meal based on genomic DNA infrared spectrum. Background technique [0002] Meat and bone meal is an important animal-derived feed, which has the characteristics of rich protein content and high biological value. It was once widely used in the field of breeding, but the subsequent outbreak of mad cow disease made meat and bone meal controlled or even banned. The European Union has issued regulations since 2001, prohibiting the addition of meat and bone meal from any source in ruminant, pig, poultry and fish feed and prohibiting the intraspecific circulation and cannibalism of animal-derived feed ingredients. Prohibition of non-ruminant sources in feed. This shows that for the sustainable development and recycling of the feed industry, scientific and reliable detection technology is an important guarantee to ens...

Claims

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Application Information

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IPC IPC(8): G01N21/35G01N21/3577C12N15/10C12Q1/6806
CPCC12N15/1003C12Q1/6806G01N21/35G01N21/3577G01N2021/3595
Inventor 刘贤韩娅红王梦妍韩鲁佳杨增玲
Owner CHINA AGRI UNIV
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