High-throughput detecting device and high-throughput detecting method combined with quantum dot fluorescence and multi-spectral camera

Abstract

The invention discloses a high-throughput detecting device and a high-throughput detecting method combined with quantum dot fluorescence and a multi-spectral camera. A power supply module is arrangedat the bottom part of a tank body; an overhead support is located above the power supply module and is fixedly arranged at the bottom part of the tank body; a control circuit board is located on the overhead support and is electrically connected with the power supply module through a guide line; a light source gathering block is located on the control circuit board and is fixedly connected with four corners of the control circuit board and the overhead support through connecting copper cylinder bolts; a multi-spectral camera module is located on the control circuit board and is located at theinner part of the light source gathering block; a test tube placement stand is fixedly arranged on the inner top surface of the tank body through an I-shaped groove formed in the inner wall of the tank body. Aiming at a method which is commonly used for quantitatively detecting biochemical products by using quantum dot fluorescence labeling, a method of using the multi-spectral camera as a fluorescence signal collector is put forward, a corresponding instrument device is designed, simultaneous detection on multiple targets and multiple samples is realized, and meanwhile, automation of a detecting process is realized.

Description

technical field [0001] The invention relates to the field of biochemical sample detection instruments, in particular to a device and method for realizing multi-target and multi-sample for biochemical detection by combining a quantum dot fluorescent labeling method and a multispectral camera. Background technique [0002] In the field of biochemical detection, methods based on optical principles occupy an important position. So far, a large number of literatures have been published using various optical methods such as absorption, scattering, surface plasmon resonance, and fluorescence for the detection of biological targets such as proteins and nucleic acids, showing unique characteristics compared with other methods. Among them, fluorescence-based detection methods have the advantages of high sensitivity, good specificity, and rapid detection, and are reported to have better detection limits than other methods. However, the detection effect of this method greatly depends o...

AI Technical Summary

Problems solved by technology

Traditional fluorescence detection devices are generally used in laboratories, and are bulky and expensive; the existing new design detection devices, due to the existence of moving parts, still have a large overall volume and mass, high power consumption, and have Reliability hidden dangers, it is still difficult for field use

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