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Toadstool strain separation method
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A technology of bacterial strain separation and Morchella, applied in directions such as botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problem of increasing the planting risk and planting cost of Morchella, the loss of growth advantage of Morchella hyphae, and success. The rate is less than 10% and other problems, so as to reduce the chance of contamination by miscellaneous bacteria, which is conducive to large-scale production and the effect of strong mycelium vitality.
Active Publication Date: 2018-11-16
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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[0003] Morchella traditionally mainly relies on the collection of wild species. In recent years, the artificial cultivation area has continued to expand, but the cultivation technology is not perfect, especially the strain separation technology, the success rate is less than 10%
Prior art is to do separation material with stipe or mushroom cap, and this part activity is poor, and separation success rate is very low; The 3rd, culture temperature is on the high side, makes hickory chick hyphae lose growth advantage relative to miscellaneous fungus hyphae, cannot effective purification
This technical shortcoming in morel strain separation increases the risk and cost of morel planting, making the strain separation technology a major bottleneck for industrial development
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Embodiment 1
[0022] In 2016, 6 morel strains were isolated in Jiangsu Xinye Ecological Agriculture Technology Co., Ltd., Pei County, Xuzhou.
[0023] The stage of selecting mother seed containers: On January 17, 10,000 special mother seed containers were ordered in Xuzhou Feichang Glass Products Co., Ltd., made of high transparent glass materials, specifications: length 15 cm, width 6 cm, height 4 cm, the diameter of the bottle mouth is 2.5 cm.
[0024] Prepare the mother seed medium: on February 8, prepare the mother seed medium;
[0025] The time to prepare the culture medium is determined by the growth of the morel fruiting body. Generally, it is separated and prepared 2-3 days in advance; use 2200 ml of purified water, 400 grams of fresh potatoes (peeled), 100 grams of wheat, 40 grams of agar, glucose 36 grams, 0.4 grams of potassium dihydrogen phosphate, 0.2 grams of magnesiumsulfate, 6 mg of vitamin B1. Prepared in a stainless steel pan. Cut 400 grams of peeled potatoes into...
Embodiment 2
[0034] In 2017, 12 morel strains were isolated in Xuzhou Guanghao Edible Fungi Professional Cooperative (Xuzhuang Village, Xuzhou Economic and Technological Development Zone).
[0035] Prepare the mother seed container: On February 11, 1,000 high-transparency glass bottles for separating the mother seed were purchased. Specifications: length 15 cm, width 6 cm, height 4 cm, bottle mouth diameter 2.5 cm.
[0036] Preparation of the mother seed medium: On February 17, the mother seed medium was prepared. Use 4400 ml of purified water, 800 grams of fresh potatoes (peeled), 200 grams of wheat, 80 grams of agar, 72 grams of glucose, 0.8 grams of potassium dihydrogen phosphate, 0.4 grams of magnesiumsulfate, 12 mg of vitamin B1; prepared in a stainless steel pot, Cut 800 grams of peeled potatoes into 0.5 cm slices, add them to water with 200 grams of wheat, boil on low heat for 15 minutes, filter, take 4000 ml of filtered juice, add 80 grams of agar, cook on low heat until com...
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Abstract
The invention discloses a toadstool strain separation method, and belongs to the field of toadstool strain separation methods. By utilizing the method, a transparent rectangular glass bottle of certain specification is taken as a mother strain container, and a culturing medium is prepared according to a certain formula. Toadstool strain mushrooms with roots having soil are whittled until maculosusbacterial contexts appear, and bacteria and soil mixtures of 0.3 millimeter squares are selected and directly inoculated to the back portion of the culturing medium plane in the container. At the certain temperature, the purification is conducted 1-2 times, and toadstool strain pure hypha bodies can be obtained. Compared with an existing toadstool strain separation method, the separation method is simple in operations, the success rate is high, and the technical bottleneck problem that the strain separation success rate is too low in the toadstool production is solved.
Description
technical field [0001] The invention relates to the field of morel strain separation methods, in particular to a morel strain separation method. Background technique [0002] Morel (Morchella deliciosa Fr.) is a rare edible and medicinal fungus. It is named for its physical pattern resembling a Morchella. It is one of the four famous fungi in the world. It is delicious, nutritious, and has high medical value. It contains 18 kinds of amino acids, of which 8 kinds of amino acids cannot be produced by the human body. It is known as the "Queen of Mushrooms". It has precious edible value and outstanding economic value. Yuan / kg or more. [0003] Traditionally, hickory chicks mainly rely on the collection of wild species. In recent years, the artificial cultivation area has continued to expand, but the cultivation technology is not yet perfect, especially the strain separation technology, with a success rate of less than 10%. The main deficiencies and defects of the existing hick...
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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 李勇史新敏樊继德李刚波赵林张婷
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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