Tung bran biotransformation microbial agent for preventing and treating citrus huanglongbing and preparation method thereof

A microbial agent and biotransformation technology, applied in biochemical equipment and methods, methods based on microorganisms, methods using microorganisms, etc., can solve the problems of citrus tree disease, death, and high morbidity, and achieve control of morbidity, green Good safety, the effect of solving the pathogenic base and the degree of disease damage

Inactive Publication Date: 2018-11-16
刘莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus is an important cultivated plant in the world, and its cultivation area and output rank among the top in the world. However, with the development of the global citrus industry, citrus diseases and insect pests have spread to major citrus production areas such as Asia, Africa, and the Americas, especially citrus greening disease. , the disease spreads quickly and has a high incidence rate, which has caused hundreds of millions of citrus trees to be infected or dead

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Take the soft soil at 15cm below the surface within the canopy of healthy old paulownia and wild healthy Citrus aurantii, dry it, pass through a 40-mesh sieve, and mix the soft soil with the acclimation medium solution containing tung tree and honey. The material ratio is 1g: 100mL, add the acclimatization medium solution containing 50g / L Tongku and 10g / L honey, shake the flask for 24h at 30°C and 200r / min, and then let it stand at 30°C Cultivate for 48 hours, remove the liquid part, then add the acclimatization medium solution containing tung cocoa and honey, repeat the cultivation for 9 times, and take the precipitated part.

[0037] (2) According to the material ratio of the precipitated part and the LB medium is 1g: 100mL, add the precipitated part into the LB medium and shake well, and separate by dilution and coating to obtain Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus pumilus and lichen For Bacillus, Bacillus subtilis, Bacillus amyloliquefaciens,...

Embodiment 2

[0040] (1) Take the soft soil at 30cm below the surface of healthy old paulownia trees and wild healthy Citrus aurantium tree canopy, dry it, pass through a 100-mesh sieve, and mix it with the acclimation medium solution containing paulownia tungii and honey. The material ratio is 1g: 100mL, add the acclimatization medium solution containing 50g / L Tongku and 10g / L honey, shake the flask for 24h at 30°C and 200r / min, and then let it stand at 30°C Cultivate for 48 hours, remove the liquid part, then add the acclimatization medium solution containing tung cocoa and honey, repeat the cultivation for 9 times, and take the precipitated part.

[0041] (2) According to the material ratio of the precipitated part and the LB medium is 1g: 100mL, add the precipitated part into the LB medium and shake well, and separate by dilution and coating to obtain Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus pumilus and lichen For Bacillus, Bacillus subtilis, Bacillus amyloliquefaciens, B...

Embodiment 3

[0044] (1) Take the soft soil at 20cm below the surface within the canopy of healthy old paulownia and wild healthy Citrus aurantii, dry it, and pass through a 50-mesh sieve, according to the ratio of the soft soil and the acclimation medium solution containing tung tree and honey. The material ratio is 1g: 100mL, add the acclimatization medium solution containing 50g / L Tongku and 10g / L honey, shake the flask for 24h at 30°C and 200r / min, and then let it stand at 30°C Cultivate for 48 hours, remove the liquid part, then add the acclimatization medium solution containing tung cocoa and honey, repeat the cultivation for 9 times, and take the precipitated part.

[0045] (2) According to the material ratio of the precipitated part and the LB medium is 1g: 100mL, add the precipitated part into the LB medium and shake well, and separate by dilution and coating to obtain Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus pumilus and lichen For Bacillus, Bacillus subtilis, Bacill...

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Abstract

The invention provides a tung bran biotransformation microbial agent for preventing and treating citrus huanglongbing and a preparation method thereof. The tung bran biotransformation microbial agentfor preventing and treating the citrus huanglongbing comprises the following steps: taking loose soils; drying and screening the loose soils; adding a domesticated culture medium solution containing tung bran and honey to the loose soils; successively shaking a bottle and performing standing for culturing; removing a liquid part; adding a domesticated culture medium solution to the loose soils torepeatedly culture the loose soils; adding a precipitate part to an LB culture medium; uniformly shaking the precipitate part and the LB culture medium; diluting, coating, separating and culturing theprecipitate part and the LB culture medium to obtain bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus and bacillus licheniformis; mixing the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus pumilus and the bacillus licheniformis to obtain a microbial strain; grafting the microbial strain to tung bran fermentation liquor containing tung bran and honey; carrying out oxygen limited fermentation on a grafted product; and filtering the fermented product to obtain the tung bran biotransformation microbial agent for preventing and treating the citrus huanglongbing. Thetung bran biotransformation microbial agent for preventing and treating the citrus huanglongbing, provided by the invention, has the advantages that the tung bran biotransformation microbial agent isprepared by using tung bran growth-promoting bacteria to ferment tung bran raw materials; and multiple effects of killing phylloxera, killing pathogenic bacteria, inducing plant wide-spectrum diseaseresistance and promoting plant inorganic nutrition and leaf photosynthesis are realized.

Description

technical field [0001] The invention belongs to the technical field of agents for preventing and treating Huanglongbing, and in particular relates to a tung tree biotransformation microbial agent for preventing and treating Huanglongbing and a preparation method thereof. Background technique [0002] Citrus is an important cultivated plant in the world, and its cultivation area and output rank among the top in the world. However, with the development of the global citrus industry, citrus diseases and insect pests have spread to major citrus production areas such as Asia, Africa, and the Americas, especially citrus greening disease. , the disease spreads quickly and has a high incidence rate, which has caused hundreds of millions of citrus trees to be infected or die. The pathogen of Huanglongbing is Phloem bacillus, a Gram-negative bacterium, most of which are round or oval, and a few are irregular, with a size of 20-600nm×170-1600nm. The outer limit of the bacteria is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20A01N63/00A01P1/00A01P3/00A01P7/04A01P21/00A01G13/00C12R1/125C12R1/07C12R1/10
CPCC12N1/36A01G13/00A01N63/00C12N1/20
Inventor 申彦秋刘莉廖祥禄
Owner 刘莉
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