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Tetraploid cultivated specie and diploid wild specie stand hybridization method for potatoes

A distant hybridization and potato technology, applied in botany equipment and methods, plant genetic improvement, grafting, etc., can solve problems such as low frequency of 2n gametes, restrictions on the development and utilization of wild potato resources, and impact on high yield

Active Publication Date: 2018-11-20
HEBEI NORTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding at the diploid level can effectively combine useful genes such as multi-resistance, but according to the potato heterosis theory, the interaction of genes affects high yield, and because tetraploid has strong heterozygosity and wide adaptability, it is enough Stable and high yield in various environments
[0007] Under natural conditions, the frequency of 2n gametes produced by wild potato species is very low, and it is limited to a few special species, which limits the development and utilization of wild potato resources

Method used

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  • Tetraploid cultivated specie and diploid wild specie stand hybridization method for potatoes
  • Tetraploid cultivated specie and diploid wild specie stand hybridization method for potatoes
  • Tetraploid cultivated specie and diploid wild specie stand hybridization method for potatoes

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Experimental program
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Effect test

Embodiment 1

[0058] 1. Test materials

[0059] Tetraploid potato: Jizhangshu No. 8 (hereinafter referred to as G8);

[0060] Diploid potato: 7 varieties of potato cultivars BD38-7, BD54-8, BD39-1, BD40-7, BD7-5, BD60-8, BD44-6.

[0061] 2. Test method

[0062] Before hybridization, by grafting method, the nutrients and genetic materials of the two are exchanged and pre-affinity. The diploid wild potato material was used as the rootstock, and the cultivated tetraploid "Ji Zhangshu No. 8" was used as the scion for grafting. 20 plants were planted for each grafting combination, 10 plants were planted in the control group, and 10 plants were planted in the wild for powder collection. use. The ungrafted varieties were used as controls. When the potato seedling grows to 2-3 mature leaves, prepare for grafting as a rootstock; when the seedling is about 3cm-5cm unearthed, prepare for grafting as a scion;

[0063] For grafting rootstocks and scions, select the upper part of wild potato twigs a...

Embodiment 2

[0076] 1. Test materials

[0077] The tetraploid potato tested was Jizhangshu 12, and the diploid potato tested were BD23-2, BD44-6, BD29-6, BD39-1, BD66-6, BD40-7, BD54-8, BD1 -5, BD7-2, BD6-8, BD38-2 a total of 11 materials.

[0078] 2. Test method

[0079] From May to August 2017, the entire experiment was carried out in the West Greenhouse of Zhangbei Test Base and the West Campus of Hebei North University. Combined with the test results of the Potato Center in 2016, the diploid resource material with high affinity with Jizhangshu 12 was grafted with it (diploid material + Jizhangshu 12). When the potato seedling grows to 2-3 mature leaves, it is used as a rootstock for grafting; when the seedling is about 3cm-5cm unearthed, it is used as a scion for grafting; the grafted body Ji Zhangshu No. 12 is the female parent, and the corresponding rootstock diploid material is The male parent, for hybridization. The ungrafted Jizhangshu 12 and the diploid resource material were...

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Abstract

The invention discloses a tetraploid cultivated specie and diploid wild specie stand hybridization method for potatoes. The method includes the steps that stocks are cultured; scions are cultured; grafting is performed; distant hybridization is performed to obtain seedling seeds, wherein the stocks are diploid wild potato species, and the scions are potato tetraploid cultivated species. The potatocultivated species and the diploid wild species are grafted into a whole and infinitely approach to each other in physiology through mutual exchange of nutrient substances and genetic substances, after the grafted tetraploid cultivated species flower as female parents, the affinity to pollen of the stock diploid wild species as male parents is enhanced, the fruit bearing percentage of hybridization is remarkably increased, and distant hybridization seeds are obtained.

Description

technical field [0001] The invention belongs to the technical field of potato distant hybridization breeding, and relates to a method for solving the difficulty in hybridizing potato tetraploid cultivated species and diploid wild species to produce seeds. This method is applicable to units, companies and individuals engaged in plant scientific research, breeding and production. Background technique [0002] Potato is the third most important food crop in the world, and was listed as the fourth largest staple food crop in my country in 2015. However, the yield per unit area of ​​potato in my country is lower than the world average level, and the increase of yield depends on excellent varieties. Potato tetraploid cultivars are used in production. It has the characteristics of narrow genetic background, poor resistance gene pool and close genetic relationship, so it is particularly important to broaden the genetic basis of potato breeding. [0003] Potatoes are native to the An...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G2/35A01G2/38A01H1/02
CPCA01H1/02A01G2/30A01G2/35A01G2/38A01H1/027
Inventor 王宽祁利潘王磊冯琰罗亚婷王燕刘畅苏云孙明磊龚学臣尹江
Owner HEBEI NORTH UNIV