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Novel application of wild rose glycoside

A technology of rosin, toxic and side effects, applied in the field of medicine, can solve the problem of difficult to effectively treat cancer, and achieve the effects of improving cell survival rate, enhancing physique, and improving cell morphology

Active Publication Date: 2018-11-20
广西英路维特药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgery and radiotherapy are local treatments, which are only effective for tumors at the treatment site. For potential metastatic lesions (cancer cells have actually metastasized, but cannot be found and detected clinically due to the limitations of current technical means) and clinical metastases cancers are difficult to treat effectively
There are no reports on the effects of rosin on the toxic side effects of cytotoxic chemotherapy drugs

Method used

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  • Novel application of wild rose glycoside
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  • Novel application of wild rose glycoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Identification of cell proliferation activity

[0039] 1. Cell culture

[0040] Both human embryonic kidney cells (HEK-293T) and mouse sarcoma cells (S180) were treated with RPMI-1640 complete medium (containing 100 μg / ml streptomycin and 100 IU / ml penicillin) containing 10% fetal bovine serum by volume fraction, Place at 37°C, 5% CO 2 Cultured in a constant temperature incubator. The cells were digested with digestive solution (0.25% trypsin) and then passaged. The cells were passaged for more than three generations, and the cells in good condition were taken for future use.

[0041] 2. MTT colorimetric method to detect cell proliferation activity

[0042] 2.1 Establishment of cisplatin, doxorubicin, paclitaxel and camptothecin injury model

[0043] (1) Take HEK-293T cells in the logarithmic growth phase, adjust the concentration of the cell suspension to 1×10 with RPMI-1640 complete medium containing 10% fetal bovine serum 5 cells / mL, mix well and inoc...

Embodiment 2

[0063] Example 2. Morphological changes of HEK-293 cells observed by inverted microscope

[0064] Take HEK-293T cells in the logarithmic growth phase, adjust the cell suspension concentration to 2×10 with RPMI-1640 complete medium containing 10% fetal bovine serum 5 cells / mL, inoculate in a 6-well plate after mixing, 2 mL / well, in 5% CO 2 , Cultured in a 37°C incubator. After the cells were cultured for 24 hours, the experiments were performed in random groups. The groups were divided into: cisplatin damage model group (100 μM); cisplatin + rosin group (100 μM + 50 μM, 100 μM + 100 μM), rosin was given 1 hour in advance; normal control group, the same volume of medium was added. Each group had 3 replicate wells, in 5% CO 2 , Continue culturing in a 37°C incubator. After culturing for 24 hours, observe the changes of cell morphology in each group under an inverted microscope, take pictures with Olympus Image-ProPlus6.0 software and conduct comparative analysis. The result ...

Embodiment 3

[0066] Example 3. The effect of rosin on the survival rate of balb / c mice induced by cisplatin

[0067] Experimental method: 8-week-old Balb / c male mice were randomly divided into four groups, and the grouping conditions and treatments were as follows: ① Control group (n=10): hydroxypropyl-β-cyclodextrin solution was given by intragastric administration every day ; ②Cisplatin model group (n=12): cisplatin (20mg kg-1) was given by intraperitoneal injection once, and hydroxypropyl-β-cyclodextrin solution was given by intragastric administration every day for 6 consecutive days. Animal death situation; ③ cisplatin + low-dose rosin group (n=12): after one-time intraperitoneal injection of cisplatin (20mg kg-1), intragastric administration of hydroxypropyl-β-cyclodextrin package every day Combined rosin (6mg kg) was administered continuously for 6 days, and the animal death situation was observed every day; ④ Cisplatin + high-dose rosin group (n=12): one-time intraperitoneal inject...

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Abstract

The invention belongs to the technical field of medicines. The invention provides novel application of wild rose glycoside, in particularly application of the wild rose glycoside to preparation of medicines for reducing toxic and side effects of cytotoxic chemotherapeutic medicines. Experiments show that the wild rose glycoside can improve the cell viability of cytotoxic chemotherapeutic medicine-damaged cells, improve the cell morphology of the cytotoxic chemotherapeutic medicine-damaged cells, and improve organ damage caused by the cytotoxic chemotherapeutic medicines; meanwhile, after combined use of the wild rose glycoside and the cytotoxic chemotherapeutic medicines, the antitumor medicinal effects of the cytotoxic chemotherapeutic medicines are not weakened. Experiments indicate thatthe wild rose glycoside can reduce the toxic and side effects of the cytotoxic chemotherapeutic medicines, improve tolerance of tumor patients to chemotherapy, improve the life quality of the tumor patients, enhance the physique and prolong the lifetime.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the new application of rosin, especially the application of rosin in the preparation of drugs for reducing the toxic and side effects of cytotoxic chemotherapy drugs. Background technique [0002] The Tibetan medicine Juema is the enlarged root tuber of the plant Potentilla anserina L. in the family Rosaceae (Potentilla L.), also known as ginseng fruit. Its wild resources are mainly distributed in the western regions of my country, with the best quality in Gansu, Qinghai, Tibet and other producing areas. Fern hemp has a sweet taste and good nature. It has the effects of promoting body fluid and quenching thirst, invigorating the spleen and stomach, nourishing qi and blood, and has great nutritional and medicinal value. [0003] Modern pharmacological studies have shown that Bracken has anti-oxidation, anti-aging, anti-virus, anti-hyperlipidemia, liver protection and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P39/02A61P35/00A61K33/24A61K31/337A61K31/4745A61K31/704
CPCA61K31/337A61K31/4745A61K31/7024A61K31/704A61K33/24A61P35/00A61P39/02A61K2300/00
Inventor 刘艳丽康乃馨许琼明李笑然杨世林
Owner 广西英路维特药物有限公司
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