A method for accurate identification of Phnom Penh carp using mitochondrial nadh5 gene

Abstract

The invention discloses a method for accurately identifying Phnom Penh carp by using the mitochondrial NADH5 gene. The method includes the following contents: (1) extracting the DNA of the Phnom Penh carp to be identified; (2) performing PCR amplification on the individual DNA template of the Phnom Penh carp by using primers; (3) Recover the amplified products; (4) Clone and sequence the PCR amplified products of the primers; (5) Compare and analyze the sequences. The present invention uses the mitochondrial NADH5 gene to identify the Phnom Penh carp, and finds that the NADH5 gene sequence of the Phnom Penh carp is 99% similar to the publicly submitted carp sequence, that is, the Phnom Penh carp belongs to a kind of carp; The bases are A and C, respectively, which are different from the G and T bases of other carp corresponding sites, so that the golden carp can be accurately identified. Using the method of the invention to identify Phnom Penh carp makes up for the deficiency of morphological identification, and provides a reference for the protection of local species of Phnom Penh carp and the settlement of commercial disputes of cultured species.

Description

technical field [0001] The invention belongs to the field of biotechnology for identifying fish species, and specifically relates to an accurate method for identifying Phnom Penh carp and other carp, which is actually a method for accurately identifying Phnom Penh carp by utilizing the mitochondrial NADH5 gene. Background technique [0002] Scientific name of carp: Cyprinus carpio . It is native to Asia and later introduced to Europe, North America and other regions. It is omnivorous. The scales are large, and there are two whiskers on each side of the palate. They live alone or in small groups in calm mud-bottom ponds, lakes, and rivers overgrown with water and grass. From the perspective of nutritional value, the protein content of carp is not only high, but also of good quality. The human body's digestion and absorption rate can reach 96%, and it can supply essential amino acids, minerals, vitamin A and vitamin D; every 100g of meat contains protein. 17.6g, 4.1g of fat...

AI Technical Summary

Problems solved by technology

However, only about 10% of the individuals in this population have golden skin from the upper edge of the head to the upper edge of the tail, which forms a thick golden line continuously or intermittently, like a "golden edge" on the back of the fish, and the remaining 90% of the individuals The shape of the carp is similar to many geographical populations and artificially selected species of carp, especially at the species stage, it is difficult to identify through the shape difference. Provide accurate identification basis for germplasm identification and disputes arising from commercial breeding

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