A kit and system for predicting susceptibility to oral cavity and head and neck malignancies
A malignant tumor, head and neck technology, applied in the field of biomedicine, can solve problems such as unscientific, inability to early warning of tumor susceptibility, impossibility, etc.
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Embodiment 1
[0109] Example 1 A system for predicting the susceptibility to malignant tumors of the oral cavity and head and neck
[0110] An oral and head and neck cancer susceptibility prediction system, comprising:
[0111] A device for obtaining the number of repetitions of the short tandem sequence of the STR site of the sample DNA;
[0112] Data processing and determination device, which includes the following modules (such as figure 1 ):
[0113] The data input module is used to input the age, gender, and repetition times of the short tandem sequence of the STR locus;
[0114] The database management module is used for the operation management of data storage, modification, deletion, query and printing;
[0115] The data calculation module is used to calculate the discriminant function result according to the repetition times of the short tandem sequence of the STR site in the data input module;
[0116] The analysis, discrimination and result output module is used to compare t...
Embodiment 2
[0152] Example 2 A kit for predicting susceptibility to oral cavity and head and neck malignancies
[0153] A kit for predicting susceptibility to oral cavity and head and neck malignancies, comprising the following components: STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers , PCR amplification reaction solution, LIZ-500 molecular weight internal standard, deionized formamide, instruction manual.
[0154] The concentrations of the above STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers are all 10 μM, and the primer sequences are shown in the following table:
[0155]
[0156] The PCR amplification reaction solution is a mixture of the following reagents: TaqDNA polymerase (5U / μL), Tris-HCl (100mM, pH 8.8 at 25°C), KCl (500mM), ethylphenyl polyethylene glycol (0.8 %(v / v)), MgCl 2 (25mM), dNTP (10mM), deionized water.
[0157] The PCR amplification reaction solution is stored at -20°C; ...
Embodiment 3
[0159] Example 3 Using the system of Example 1 and the kit of Example 2 to predict the risk of the subject suffering from oral cavity and head and neck cancer
[0160] Subject: Male, 64 years old, visited the Department of Otorhinolaryngology, China-Japan Friendship Hospital of Jilin University. After fully informing the purpose and purpose of the examination, and on the premise of his willingness, he signed an informed consent and collected 1mL of anticoagulant blood through a peripheral vein. .
[0161] Use the kit of embodiment 2, carry out the following steps according to the method recorded on the kit instruction manual:
[0162] (1) Extract sample DNA: Use a DNA extraction kit to extract blood genomic DNA;
[0163] (2) PCR reaction
[0164] (2-1) Take out STR-1 primer, STR-2 primer, STR-3 primer, STR-4 primer, STR-5 primer, STR-6 primer, PCR amplification reaction solution from the refrigerator, equilibrate to room temperature, each After the components are fully diss...
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