NSCLC (non-small cell lung cancer) detection kit and application thereof

A non-small cell lung cancer, expression level technology, applied in the field of non-small cell lung cancer detection kits, can solve the problems of large changes in the diagnostic value of miRNA and large differences in markers, etc.

Active Publication Date: 2018-11-20
CAPITALBIO CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, different research groups mostly use a single reference miRNA for data normalization, and the markers screened by different NSCLC studies are quite different, and the diagnostic value of some miRNAs varies g

Method used

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  • NSCLC (non-small cell lung cancer) detection kit and application thereof
  • NSCLC (non-small cell lung cancer) detection kit and application thereof
  • NSCLC (non-small cell lung cancer) detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, 9 kinds of miRNA can be used for the diagnosis of non-small cell lung cancer

[0053] 1. Collection of samples and collation of sample data

[0054] The inventor of the present application collected plasma samples that meet the standards according to the standard operating procedure (SOP), systematically collected complete demographic data, clinical data, etc., and through sorting out the sample data, the inventor selected the plasma samples of 1375 people as miRNA Chip detection and subsequent series of qRT-PCR verification experimental samples, these 1375 people were divided into the following four groups:

[0055] Non-small cell lung cancer group: including 462 patients with non-small cell lung cancer. The inclusion criteria for this group are: patients with newly diagnosed and untreated lung adenocarcinoma or lung squamous cell carcinoma diagnosed by pathology, and patients who have not undergone surgery and lung cancer before blood collection. Chemor...

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Abstract

The invention discloses an NSCLC (non-small cell lung cancer) detection kit and an application thereof. The NSCLC detection kit comprises substances for detecting the content of 9 miRNAs (miRNA-hsa-miR-20a, hsa-miR-25, hsa-miR-223, hsa-miR-320, hsa-miR-222, hsa-miR-146a, hsa-miR-191, hsa-miR-24 and hsa-miR-130a), CEA and CYFRA21-1. The kit can be used for well screening NSCLC and is high in sensitivity, high in specificity and suitable for screening of NSCLC, particularly for NSCLC at the early stage. Therefore, early warning can be given for the lung cancer risk of people by use of the 9 miRNAs in combination with the CEA and CYFRA21-1, and the early diagnosis proportion can be increased.

Description

technical field [0001] The invention relates to a non-small cell lung cancer detection kit and its application in the field of biomedicine. Background technique [0002] Lung cancer is one of the most common malignant tumors clinically. Lung cancer is mainly divided into small cell lung cancer and non-small cell lung cancer. The main type of lung cancer in my country is non-small cell lung cancer, accounting for about 80% of lung cancer patients, mainly including two types of adenocarcinoma and squamous cell carcinoma. Early detection and early treatment of lung cancer patients are very likely to improve the survival rate and reduce mortality of lung cancer patients. [0003] At present, the commonly used diagnosis of lung cancer mainly relies on the finding of intrapulmonary tumors on imaging, including chest X-ray, CT, nuclear magnetic resonance (MRI) and other imaging methods, combined with tumor markers such as carcinoembryonic antigen in serum. However, imaging exami...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 郭弘妍杨春花王俊杨帆王亚辉邢婉丽程京
Owner CAPITALBIO CORP
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