Multi-beam confocal high-speed scanning imaging method and device based on polyhedral prism
A high-speed scanning and polyhedron technology, applied in the field of optics, can solve problems such as the inability to effectively distinguish between multi-focus and multi-focus excited fluorescence signals, and achieve the effect of increasing the frame rate
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Embodiment 1
[0042] The device for generating array multi-focus based on polyhedral prism includes focusing objective lens 2, beam conversion lens unit, two-dimensional galvanometer 5, dichroic mirror 6, focusing lens unit, array detector 8 and polyhedral prism. The focusing lens unit includes a lens or A combination of multiple lenses; the polyhedral prism is a cylinder, and its cross-section is a polygon with a base and multiple edges. The direction of the beam g is perpendicular to the base and the beam g enters the base first. The number of focused spots and the edges The role of the polyhedral prism is to generate multiple parallel beams with a certain angle with the optical axis (y-axis).
[0043] In this embodiment, the beam conversion lens unit includes lens 3 and lens 4, the focusing lens unit is a single lens 7, and the polyhedral prism is a polyhedral prism. figure 2 The prism 9 shown.
[0044] Such as figure 1 As shown, the prism 9, the dichroic mirror 6, and the two-dimensional ga...
Embodiment 2
[0066] Such as figure 1 As shown, if the angles between the two facets m1 and m2 of the prism 9 and the z-axis are θ 1 And θ 2 , The refractive index of the prism 9 is n, then the angle between the refracted light of the prism face m1 and the first optical axis (y-axis) is θ 1 ′=asin(nsinθ 1 -θ 1 ),Such as Figure 4 As shown, the distance h from the focused spot A to the second optical axis (x axis) 1 =fsin[asin(nsinθ 1 -θ 1 )], where f is the focal length of the focusing objective lens 2. Similarly, the distance from the focused spot B to the second optical axis h 2 =fsin[asin(nsinθ 2 -θ 2 )]. Therefore, the position of the focus spot can be precisely controlled by the refractive index of the prism n, the angle between the prism faces m1 and m2 and the z axis, and the focal length f of the focusing lens 2. If the planes m1 and m2 are asymmetric about the first optical axis, the intensity of the focused spot A and the focused spot B are different. The intensity of the focused s...
Embodiment 3
[0068] This embodiment is the same as the other structures and settings in the first embodiment, except that the polyhedral prism is replaced in this embodiment as Figure 5 The polyhedral prism shown on the center left. The polyhedral prism is a tetrahedral cylindrical prism with a bottom surface and three prism surfaces, and the three prism surfaces are symmetrically arranged along the first optical axis.
[0069] In this embodiment, the focused spot distribution is as follows: Figure 5 The three focus spots on the right side are arranged along the y axis on the xy plane. If the prism surface is perpendicular to the light beam g and symmetrically arranged along the first optical axis, the focused spot obtained through the prism surface is on the origin of the coordinate axis.
[0070] Further, assuming that the confocal scanning imaging will finally obtain an image with n rows and n columns, using the polyhedral prism of this embodiment to generate a three-point column distribut...
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