Method for culturing monoecism taxus chinensis through micro-grafting
A technology of monoecious plants and micro-grafting, which is applied in the field of plant cultivation to achieve the effect of improving the ornamental effect
Active Publication Date: 2018-11-23
BEIHUA UNIV
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Problems solved by technology
[0004] In order to solve the above-mentioned problems in the prior art, the present invention provides a method for cultivating monoecious Taxus chinensis by micro-grafting, so as to solve the problem that a single plant cannot bear fruit
Method used
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Embodiment 1
[0061] In this example, select female Northeast yew plants, cut off 1-year-old branches with a length of 4 to 5 cm, and keep 3 to 5 small branches, dip them in ABT rooting powder quickly, and cut them. The cutting substrate is humus, and the temperature is controlled at 20±2°C , relative humidity 90-100%, induce adventitious roots.
[0062] Cultivate for 6 to 7 weeks, and take root one after another. When the root length is 0.3 to 0.5 cm, sterilize on the inner surface of the ultra-clean workbench in the sterile room, and transplant it into the pre-prepared MS medium under aseptic conditions, and put it on the culture medium. Indoor cultivation.
[0063] At the same time, male plant branches with the same diameter were selected, sterilized on the inner surface of the ultra-clean workbench in the sterile room, inoculated into the pre-prepared MS medium under sterile conditions, and placed in the cultivation room to cultivate the scion. Female seedlings and male scions were cul...
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The invention discloses a method for culturing monoecism taxus chinensis through micro-grafting. The method comprises the steps of conducting primary culturing, wherein female plant saplings and maleplant branches are inoculated to culture bottles loaded with a first culture medium and a second culture medium respectively to be cultured for 1-2 weeks; conducting micro-grafting, wherein at least one branch apical bud is cut off from female plant stocks; male plant scions are taken out, base parts to are cut to be flat, and the male plant scions are grafted to branches of the female plant saplings of which apical buds are cut off; conducting management after grafting; wherein the culture bottles are sealed, the female plant stocks grafted with the male scions continue to be cultured in theculture bottle loaded with the first culture medium for 3-4 weeks, healing of grafted notches is promoted, and grafted saplings are obtained; transplanting the grafted saplings into a humus medium, putting the grafted saplings in a greenhouse to be subjected to habituated culture for 3-4 weeks, and conducting culturing to obtain monoecism taxus chinensis saplings. The monoecism taxus chinensis saplings cultured by means of the method for culturing monoecism taxus chinensis through micro-grafting have both female flower branches and male flower branches, monoecism of taxus chinensis is achieved, and the ornamental value is increased.
Description
technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for cultivating monoecious yews by micro-body grafting. Background technique [0002] Northeast yew (Taxus cuspidata) belongs to the family Taxaceae (Taxaceae), and the genus Taxus (Taxus) is an evergreen coniferous tree. Northeast yew is recognized as an endangered natural and rare anti-cancer plant in the world. It has a history of 2.5 million years on the earth and is a living fossil of plants. In 1996, UNESCO listed it as a rare and endangered plant in the world; in 1999, it was listed as a first-class rare and endangered wild plant in my country. [0003] Greening and ornamental is one of the uses of Northeast yew. The Northeast yew has a beautiful tree shape, dense and green branches and leaves in four seasons, summer and autumn fruit viewing, and bright red berries, like rubies inlaid in huge emeralds. It is a rare evergreen ornamental tree species in Northeast C...
Claims
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IPC IPC(8): A01G2/30A01G17/00
CPCA01G2/30A01G17/005
Inventor 程广有唐晓杰任文利
Owner BEIHUA UNIV