Application of rapamycin in inhibiting plasmodium propagation

A technology of rapamycin, Plasmodium, applied in a new field of application to inhibit the transmission of Plasmodium by malaria vectors

Inactive Publication Date: 2018-11-23
FUDAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But current methods of vecto...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of rapamycin in inhibiting plasmodium propagation
  • Application of rapamycin in inhibiting plasmodium propagation
  • Application of rapamycin in inhibiting plasmodium propagation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of Rapamycin Reagent 1: Rapamycin was prepared as a 20 mM stock solution with DMSO, and the working solution was diluted 1:1000 with PBS to make the final concentration 20 μM.

[0028] Blank reagent 1: DMSO diluted 1:1000 in PBS.

[0029] The experimental group injected 69nL of 20 μM rapamycin reagent 1 into the body cavity of Anopheles mosquitoes by microinjection; 12 hours after injection, mice infected with Plasmodium were fed to Anopheles mosquitoes; 8 days later, the infection rate of Plasmodium in mosquitoes was detected . In the control group, the blank reagent 1 was used to replace the rapamycin reagent 1, and the same experimental procedure was carried out.

[0030] The results showed that the number of Plasmodium oocysts in the Anopheles mosquitoes treated with rapamycin in the experimental group was significantly reduced compared with the control group ( figure 1 ), ** means p<0.01. It shows that rapamycin can improve the anti-plasma parasite i...

Embodiment 2

[0032] Preparation of rapamycin reagent 2: Make rapamycin into a 25μg / μl stock solution with absolute ethanol, and dilute it 250 times with diluent before use to make the final concentration 0.1μg / μl; the formula of the diluent is: volume fraction 5% Tween-80, 5% polyethylene glycol, 4% ethanol, 86% deionized water.

[0033] Blank reagent 2: Absolute ethanol diluted 250 times with the same diluent.

[0034] Four days after the mice were infected with Plasmodium, the blood density of the parasites reached 3%-5%. Experimental group: The infected mice were injected with rapamycin reagent 2 through the tail vein, and the injection dose was 200 μL per 20 g of mouse body weight; 15 minutes after injection, the mice were anesthetized and fed with mosquitoes, and 8 days later, Plasmodium eggs in mosquitoes were detected. number of sacs. For the control, use blank reagent 2 instead of Pamycin reagent 2, and follow the same experimental procedure.

[0035] The results showed that in ...

Embodiment 3

[0036] Example 3 Determination of the regulatory effect of rapamycin on the immune system of Anopheles mosquitoes

[0037] Preparation of Rapamycin Reagent 1: Rapamycin was prepared as a 20 mM stock solution with DMSO, and the working solution was diluted 1:1000 with PBS to make the final concentration 20 μM.

[0038] Blank reagent 1: DMSO diluted 1:1000 in PBS.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses new application of rapamycin in inhibiting propagation of plasmodium by entomoplily. The new application is characterized in that the rapamycin is used for decreasing the number of plasmodium oocysts in an entomoplily body; the rapamycin is used for improving the antiplasmodial infection ability of the entomoplily; and the rapamycin is used for improving the expression level of immune effector molecules TEP1 of the entomoplily. The invention also discloses application of the rapamycin in preparation of a reagent for inhibiting the propagation of the plasmodium by the entomoplily and a special preparation. It is discovered that the rapamycin can improve the ability of resisting mosquito infection by improving the expression level of PET-1, can achieve the purposes ofdecreasing the number of the plasmodium oocysts in the entomoplily body and effectively inhibiting the propagation of the plasmodium by anopheles, and the rapamycin can be used as a blocking drug ofthe propagation of the plasmodium by the mosquitoes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new application of rapamycin for inhibiting the transmission of Plasmodium by a malaria transmission vector. Background technique [0002] Malaria is a global insect-borne infectious disease transmitted by Plasmodium through the bite of Anopheles mosquitoes. It is one of the key infectious diseases in the United Nations Millennium Development Goals. public health issues. Due to the lack of an effective vaccine, there is a problem of increased resistance of Plasmodium to traditional therapeutic drugs, and the control of malaria mainly relies on the prevention and control of the vector Anopheles. However, the current methods of controlling insect vectors face enormous challenges. Resistant vectors to traditional insecticides have emerged in multiple regions, and in some regions even the same vector is resistant to all four existing insecticides, so there is an urgent ne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/436A61P33/02
CPCA61K31/436A61P33/02Y02A50/30
Inventor 王敬文冯岳彪董丽
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap