Unlock instant, AI-driven research and patent intelligence for your innovation.

Cell sorting device and sorting method

A sorting device and cell technology, applied in the field of biomedical engineering, can solve the problems of death, cell damage, inability to realize living cells, etc., to reduce cell damage, improve efficiency, and achieve the effect of specific sorting

Inactive Publication Date: 2018-11-23
SUZHOU BAIYUAN GENT CO LTD
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the technical problem to be solved by the present invention is to solve the defects in the prior art that cell damage and death are easily caused when the target cells in blood are classified and screened, live cell sorting cannot be realized, and the sorting efficiency is low and the cost is high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell sorting device and sorting method
  • Cell sorting device and sorting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This embodiment provides a cell sorting device, including a fluid pipeline 1 , a cell sorting unit 2 , a cell delivery unit 3 , a magnetic field assembly 4 and a cell processing unit 5 .

[0062] Such as figure 1 As shown, the fluid pipeline 1 extends in the horizontal direction, and a first opening 11 and a second opening 12 are respectively formed at both ends of the fluid pipeline 1. The first opening 11 of the fluid pipeline 1 is connected to the sample pipeline, and the sample pipeline flows into the fluid pipeline 1. A sample solution containing target cells to be sorted, such as blood, or a mixed solution of various cells is delivered.

[0063] The cell sorting unit 2 includes three cell sorting chambers 21, the fluid pipeline 1 communicates with the three cell sorting chambers 21 in sequence, and the fluid pipeline 1 communicates with the symmetrical side walls of each cell sorting chamber 21, and An inflow port and an outflow port are respectively formed on th...

Embodiment 2

[0078] This embodiment provides a cell sorting method, which is to perform cell sorting on blood samples of clinical tumor patients, and the cell sorting device provided in Example 1 is used for cell sorting. The cell sorting method includes the following steps:

[0079] (1) Pump the blood sample into the fluid pipeline 1 through the sample pipeline, close the third control switch 25 of the first cell separation chamber 21, and the blood sample flows into the first cell separation chamber 21 connected to the fluid pipeline 1 through the fluid pipeline 1 ;

[0080] (2) The erythrocytes in the blood sample are specifically combined with the first antibody modified on the surface of the magnetic beads in the first cell sorting chamber 21, and the magnetic field assembly 4 is turned on, and the magnetic field assembly 4 generates a magnetic force so that the magnetic beads bound with erythrocytes are adsorbed on the first cell separation chamber 21. The bottom of the cell sorting...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cell sorting device which comprises a fluid pipeline, a cell sorting unit, a cell conveying unit and a magnetic field assembly, wherein the cell sorting unit comprises at least one cell sorting chamber, the fluid pipeline is sequentially communicated with the cell sorting chamber, and antibody modified magnetic beads are arranged in the cell sorting chamber; types of cells combined with antibody in the same cell sorting chamber are the same, and furthermore, types of cells combined with the antibody in at least one cell sorting chamber is different from that of the cells combined with the antibodies in another cell sorting chamber. Specific recognition and combination to different types of cells can be achieved through the magnetic beads in the cell sorting chambers; furthermore, the magnetic beads are adsorbed by magnetic force generated by the magnetic field assembly, so that target cells can be accumulated in the corresponding cell sorting chamber, and living cell sorting is achieved; the cell sorting device has the advantages of strong specificity, high flexibility and no pretreatment on a sample solution. The invention further discloses a cell sortingmethod. By means of the cell sorting device, living cells can be sorted in high efficiency.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a cell sorting device and a sorting method. Background technique [0002] The application of blood is more and more extensive, and it is often used in industries such as food, biological products, feed and clinical medicine. At present, component blood transfusion is commonly used clinically, which usually requires the separation of red blood cells and platelets. Component blood transfusion not only saves blood sources, but also has the advantages of multiple uses of one blood, strong pertinence, and easy storage and transportation. Therefore, it is efficient and safe. It is particularly important to separate the main components in the blood. [0003] The separation of blood cells in blood is usually done by centrifugation or membrane separation. The main steps of centrifugal separation of blood cells are as follows: (1) Add an appropriate amount of blood into the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/00C12M1/42C12M1/36C12M1/34C12M1/02C12N5/00C12N13/00
CPCC12M23/16C12M27/02C12M35/06C12M41/00C12M41/36C12N5/00C12N13/00
Inventor 车团结徐红李琳李亚鹏
Owner SUZHOU BAIYUAN GENT CO LTD