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A dynamic optical detection method for cell deformation and cell activity

A cell viability and optical detection technology, applied in the field of biotechnology applications, can solve the problems of high equipment prices, radionuclide environmental pollution, high cost of raw materials, etc., and achieve the effect of no toxic side effects, intuitive detection results, simple and fast operation

Active Publication Date: 2022-04-01
NINGBO MEIJING MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection result of this method is relatively accurate, but due to the need to use a specific liquid scintillation counter, the equipment price is high, the cost of raw materials is high, and radionuclides are easy to cause environmental pollution

Method used

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  • A dynamic optical detection method for cell deformation and cell activity
  • A dynamic optical detection method for cell deformation and cell activity
  • A dynamic optical detection method for cell deformation and cell activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A dynamic optical detection method for cell deformation and cell activity, comprising the following steps:

[0032] ① Prepare cell deformation culture plate 1;

[0033] ② Observe the cultured cells with a microscope to confirm that the growth state is good, remove the original medium, then trypsinize the cultured cells, add new medium to stop the digestion, centrifuge at 200g for 3 minutes, and discard the upper layer of medium ;

[0034] ③Resuspend the diluted cells with new medium, take a single cell and a certain volume of new medium, transfer to the cell deformation culture plate 1 to continue culturing, and the cultured cells in the cell deformation culture plate 1 produce different deformations according to the cell activity;

[0035] ④Use a microscope to observe the morphological changes of the same cell at different times as needed, and record the observation results;

[0036] ⑤ The deformation and mechanics of the cell have a strong correlation with cell phys...

Embodiment 2

[0046] The rest are the same as in Example 1, except that: 2. Centrifuge for 5 minutes under 150g centrifugal force, and discard the medium in the upper layer;

Embodiment 3

[0048] The rest are the same as in Example 1, except that: 2. Centrifuge for 2 minutes under 250g centrifugal force, and discard the medium in the upper layer;

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Abstract

The invention discloses a dynamic optical detection method for cell deformation and cell activity, which is characterized by: preparing a cell deformation culture plate; observing the cultured cells with a microscope to confirm that the growth state is good, and then digesting the cultured cells with trypsin After centrifugation, discard the original medium on the upper layer; resuspend the diluted cells with new medium, take a single cell and a certain volume of new medium, transfer to a cell deformation culture plate to continue culturing, and the cultured cells are placed in a cell deformation culture plate Different deformations are produced according to different cell activities; according to the needs, use a microscope to observe the morphological changes of the same cell at different times, record the observation results, and analyze the cell activity; the advantages are: the method of the present invention is simple and quick to operate, and can be continuous, dynamic and intuitive It can accurately monitor the deformation and activity characteristics of cells in different time periods.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to a dynamic optical detection method for cell deformation and cell activity. Background technique [0002] Cell activity is an important indicator for judging whether cells cultured in vitro can grow normally under conditions such as drug treatment, radiation or ultraviolet radiation, and changes in culture conditions. The detection of cell viability is a common method for relevant scientific research, and it is also one of the important methods for in vitro screening of anti-tumor drugs and clinical tumor drug sensitivity tests. There are many commonly used methods for detecting cell viability, such as staining, colony (colony) formation, colorimetry, and radioisotope incorporation. [0003] Staining method is the most commonly used method to check cell life and death in cell culture. It is divided into chemical staining method and fluorescent staining method, which uses ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5011G01N33/5026
Inventor 张晓晶沈挺宋毅李春燕
Owner NINGBO MEIJING MEDICAL TECH