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Solid-phase extraction column for enriching and purifying mycotoxin and preparation method thereof

A technology of solid-phase extraction columns and mycotoxins, which is applied in the preparation of test samples, chemical instruments and methods, separation methods, etc., can solve the problems of expensive immunoaffinity columns, etc., and achieve stable purification methods, good adsorption performance, cost-saving effect

Inactive Publication Date: 2018-11-27
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the commercialized immunoaffinity column is very expensive, and the price of a single column even reaches 150-180 yuan, and it only targets a certain type or type of toxin

Method used

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  • Solid-phase extraction column for enriching and purifying mycotoxin and preparation method thereof
  • Solid-phase extraction column for enriching and purifying mycotoxin and preparation method thereof
  • Solid-phase extraction column for enriching and purifying mycotoxin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Enrichment and purification of nine kinds of mycotoxins in milk

[0036] (1) Preparation of graphene alloy composite material:

[0037] The first step is to prepare the gold nanoparticle solution: accurately measure 0.5mL of tetrachloroauric acid trihydrate (0.45g / 45mL) solution and 2.5mL of L-ascorbic acid solution (0.4g / L), and mix the two solutions to 50mL deionized water for 2 min at room temperature. Then 0.5 mL of 1% sodium citrate aqueous solution was added, and the reaction was stopped after 9 minutes, and centrifuged at 13000 rpm for 5 minutes. Finally, 20 mL of water redissolved the residue, and ultrasonically oscillated to prepare a gold nanoparticle solution;

[0038] The second step is to prepare reduced graphene oxide: accurately weigh 25.11 mg of graphene oxide (GO), dissolve it in 50 mL of water and add NaOH to adjust the pH to 10, then add 29 mg of L-ascorbic acid, and stir at 80 ° C for 4 hours , and then the obtained black dispersion was...

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PUM

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Abstract

The invention discloses a solid-phase extraction column for enriching and purifying mycotoxin in milk. The solid-phase extraction column is prepared from a graphene alloy composite material as a filling. The solid-phase extraction column can enrich nine types of mycotoxin in milk simultaneously, is cheap and is simple to operate.

Description

technical field [0001] The invention relates to the technical field of safety detection of agricultural and sideline products, in particular to a solid-phase extraction column for enriching and purifying mycotoxins and a preparation method thereof. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by fungi, which have cytotoxicity, immunotoxicity, reproductive toxicity, neurotoxicity and triple (carcinogenic, teratogenic and mutagenic) effects on humans and animals. Mycotoxins not only contaminate feed and crops, but also transfer toxins and their metabolites to animal-derived foods (such as milk, meat, etc.) through animals that ingest contaminated feed, which is one of the important factors affecting their safety . Aflatoxin B 1 (AFB 1 ), aflatoxin M 1 (AFM 1 ), ochratoxin A (OTA), zearalenone (ZEA), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalenone ( ZAN), α-zearallanol (α-ZAL) and β-zearalanol (β-ZAL) nine mycotoxins have been ...

Claims

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Application Information

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IPC IPC(8): B01D15/20B01J20/282B01J20/30G01N1/40
CPCB01D15/20B01J20/282G01N1/4055G01N2001/4061
Inventor 韩铮吴立冬赵志辉聂冬霞郭文博黄晴雯蒋可秋
Owner SHANGHAI ACAD OF AGRI SCI
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