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Separating method for wild lactarius deliciosus strains

A technology of wild pine milk mushroom seed and separation method, which is applied in the field of acquisition and application of wild pine milk mushroom strains, can solve the problem of lack of domestication and cultivation of edible fungi, weak growth speed or activity, and pine milk mushroom It can achieve the effect of fast growth, easy germination and avoiding pollution.

Inactive Publication Date: 2018-11-30
上海沃施创业孵化器管理有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult to successfully isolate the strains of Pine Milk Mushroom at present. Even if the separation is successful, its growth rate or activity is not strong.

Method used

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  • Separating method for wild lactarius deliciosus strains

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Embodiment 1

[0025] A method for isolating wild pine milk mushroom strains, comprising:

[0026] (1) Preparation of the first medium: the first medium contains 20g / L Fagaceae chestnut water extract, 20g / L agar, 20g / L glucose, 0.1g / L calcium chloride, 0.1g / L L magnesium sulfate, 0.8g / L potassium dihydrogen phosphate, 0.01g / L sodium chloride, 0.2g / L diammonium hydrogen phosphate, 0.1g / L ferric chloride, 0.1g / L zinc sulfate, 0.05g / L sulfuric acid Copper, 0.01g / L Niacin, 0.1mg / L Vitamin B 1 And 1g / L wort, its preparation method is: add the dry leaf of Fagaceae chestnut into water, the weight ratio of the dry leaf of Fagaceae chestnut to water is 1:100, filter after boiling for 10min, the obtained filtrate It is the water extract of Fagaceae plants. Add agar to the filtrate in proportion and heat it to melt. After the agar is completely melted, add medicines one by one. The medicines include glucose, calcium chloride, magnesium sulfate, potassium dihydrogen phosphate, and sodium chloride. , d...

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Abstract

The invention relates to a separating method for wild lactarius deliciosus strains. The separating method for the wild lactarius deliciosus strains is characterized by comprising the following steps:collecting sporocarps of wild lactarius deliciosus, separating gills, performing cultivation in a first culture medium, purifying the obtained strains, transferring the purified strains to an ordinaryculture medium, and performing adaptive cultivation to obtain a wild lactarius deliciosus strain. According to the method provided by the invention, the wild lactarius deliciosus strain obtained by the method has the advantages of strong activity and a fast growth speed, and the separating method has uniqueness in separating methods for the wild lactarius deliciosus strains in China.

Description

technical field [0001] The invention belongs to the field of acquisition and application of pine milk mushroom strains, in particular relates to the acquisition and application of wild pine milk mushroom strains in Zhejiang. Background technique [0002] Pine milk mushroom (Lactarius deliciosus) belongs to Russulaceae and the genus Lactarius, also known as delicious pine mushroom, pine mushroom, pine mushroom, fir tree mushroom, and thatch mushroom. It is a very popular delicious edible fungus. my country is one of the countries with particularly rich biodiversity of wild mushrooms. It is estimated that there are 100,000 species of edible and medicinal fungi in China, but only about 1,000 species have been known, and only about 100 species have been used. It can be seen that most wild edible fungi Medicinal fungi have not been discovered, recognized and utilized. Domestication and cultivation of better edible and medicinal fungi is one of the important tasks of edible and me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 吴海林郭桂飞
Owner 上海沃施创业孵化器管理有限公司