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Establishing method for retina edema animal model

A technology of retinal edema and establishment method, which is applied in the field of establishment of retinal edema animal models, can solve the problems of retinal atrophy, vascular damage to the retina, and increased retinal damage, and achieve the effect of small trauma and good repeatability

Active Publication Date: 2018-11-30
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars prepared a retinal edema model by occluding retinal veins: SD rats were selected, and the photosensitizer Erythrosin B was injected intravenously combined with 532nm laser to continuously irradiate the retina for 8 minutes. Vascular damage and retinal thickness increased immediately after the operation, but the retinal thickness increased 5 days after the operation. After recovery to pre-operation, retinal atrophy occurred 14 days later, and it was impossible to provide an animal model of long-term retinal edema, and long-term laser irradiation increased damage to other parts of the retina

Method used

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  • Establishing method for retina edema animal model
  • Establishing method for retina edema animal model
  • Establishing method for retina edema animal model

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Example 1: Using BN rats to establish a retinal edema model

[0047] Step 1: Preparation of Rose Bengal Solution

[0048] Under dark conditions, accurately weigh 50 mg of Rose Bengal powder, place it in a 1.5 ml brown EP tube, add 1.0 ml of normal saline, and mix well. After filtering 3 times with a 0.22μm sterile filter, the prepared Rose Bengal solution (50mg / ml) was stored at 4°C for later use.

[0049] Step 2: BN rat preparation

[0050] Four female BN rats, 7-8 weeks old, weighing an average of 150 g, were injected intraperitoneally with 10% (w / w) chloral hydrate (0.3 ml / 100 g). After the anesthesia is completed, the compound tropikamide eye drops are fully dilated, slit lamp, OCTA / OCT examination, and recording ( figure 2 ). The rat is fixed and the tail is fully disinfected with 75% alcohol. 50mg / ml rose bengal solution (0.1ml / 100g) was allowed to stand at room temperature for 10 minutes and then injected into the rat tail vein. After the injection, it was disinfecte...

Embodiment 2

[0058] Example 2: Using C57BL / 6 mice to establish a retinal edema model

[0059] Step 1: Preparation of Rose Bengal Solution

[0060] Under dark conditions, accurately weigh 25 mg of rose bengal powder, place it in a 1.5 ml brown EP tube, add 1.0 ml of physiological saline, and mix well. After filtering 3 times with a 0.22μm sterile filter, a Rose Bengal solution (25mg / ml) was prepared and stored at 4°C for later use.

[0061] Step 2: Preparation of C57BL / 6 mice

[0062] 15 female C57BL / 6 mice, 6-8 weeks old, average weight 20g, 10% (w / w) chloral hydrate (1ml / 100g) intraperitoneally injected, after anesthesia, compound tropicamide eye drops are sufficient Mydriasis, slit lamp, OCTA and OCT examination, record. Fix the mice, and fully disinfect the tail with 75% alcohol. 25mg / ml Bengal Rose Solution (0.5ml / 100g) was left to stand at room temperature for 10 minutes and then injected into the rat tail vein. After the injection, it was disinfected with alcohol again to prevent infectio...

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Abstract

The invention discloses an establishing method for a retinal edema animal model. A reasonable application of 577 nm laser combined with rose Bengal is used to block a retinal vein of a mouse to form retinal edema, and a non-invasive OCTA / OCT technique is used to continuously observe a retina of a model eye and edema states of the retina. The invention aims to invent the establishing method with small trauma to model animals, rapid onset effect, long maintenance time and simple and easy operation for the retinal edema animal model for meeting the corresponding experimental requirements well.

Description

Technical field [0001] The invention relates to a method for establishing an animal model of retinal edema. Background technique [0002] As a common eye disease, macular edema is more common in retinal vein occlusion, diabetic retinopathy, chronic uveitis, ocular trauma, and after intraocular surgery. The patient's conscious visual acuity decreases, and his vision becomes worse, which seriously affects the patient's quality of life. The pathogenesis of macular edema is mainly due to the leakage of capillary damage in the macular area caused by the above-mentioned primary diseases. At present, a large number of scientific researchers are committed to the research and development of drugs for the treatment of macular edema. Pre-clinical experiments require an animal model of retinal edema to observe the therapeutic effects of the drugs developed. The establishment of this model requires simplicity, low-invasiveness, fast onset and long maintenance time. [0003] According to rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61D7/00
CPCA01K67/02A61D7/00
Inventor 曲毅程同杰程英李靳
Owner SHANDONG UNIV QILU HOSPITAL
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