A method for raising seedlings of rice with resistance to stress in saline-alkali cold regions
A technology for rice and cold areas, which is applied in the field of plant seedlings, can solve the problems of raising seedlings that do not involve the stress resistance of rice in saline-alkali soil in cold areas, and do not involve salt and alkali tolerance, so as to improve germination ability, seedling growth, and stress resistance The effect of wide applicability and nutrient degree and substrate
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preparation example 1
[0057] Preparation of fucoidan extract
[0058] Take 1kg of fresh brown algae, wash and pulverize it, add 10L of deionized water, and beat it in a beater to obtain a slurry; add 600mg of acid cellulase to the slurry, adjust the pH to 5.0, and place it in a water bath at 42°C to slowly stir for enzymolysis 6h; After the cellulase enzymolysis, add 300mg of alkaline pectinase to the enzymolysis solution, enzymolysis at pH 9.0, 50°C water bath for 8h, then boil to kill the enzyme, press filter to remove solid impurities, adjust the pH to 6.8 , so as to obtain the final enzymolysis solution; the enzymolysis solution is heated and concentrated, evaporated to remove water, the obtained solid is dried and ultrafinely pulverized, so as to obtain the brown algae extract containing the main component of polysaccharide.
preparation example 2
[0060] Preparation of Rapeseed Pollen Extract
[0061] Take 100 grams of dried rapeseed pollen, add 500 mL of 75% ethanol to extract for 2 hours, during the extraction period, 200-300w ultrasonic assisted treatment for 20 minutes is used to speed up the extraction, after the alcohol extraction is completed, filter, and the filtrate is vacuum concentrated to 100ml to obtain a concentrated alcohol extract. At the same time recover the ethanol solvent; extract the ethanol extract twice with 400mL ethyl acetate in the extraction bottle, combine the ethyl acetate extract layer and concentrate to 30ml, then add the enzyme solution consisting of 50ml of neutral lipase and 50ml of amylase respectively 100ml of compound enzyme solution, which contains 100U / L of neutral lipase and 60U / L of amylase in the compound enzyme solution. The enzymolysis solution was slowly stirred at 42°C for 1.5 hours, then heated to inactivate the enzyme, and recovered by rotary steaming Vacuum drying after s...
Embodiment 1
[0063] A method for stress-resistant drought seedling raising of rice in saline-alkali soil in cold regions, the specific steps are as follows:
[0064] S1: Treat rice seeds with seed soaking and coating agent:
[0065] Prepare 100ml of stock solution of seed soaking and coating agent. The stock solution of seed soaking and coating agent includes the following components: 10wt% of fucoidan mixed extract, 2wt% of rape flower extract, 3wt% of betaine, and 0.05wt% of potassium fulvic acid , 0.01wt% of β-aminobutyric acid, 0.001wt% of sodium nitrophenolate, and the balance is water; the above-mentioned seed soaking and coating agent solution is diluted 10 times with deionized water to obtain a dilution, and the dilution weight ratio of 0.5wt% is added Sophorolipids, stirred evenly to obtain seed soaking liquid.
[0066] Use the above seed soaking solution to soak the screened full-grained and high-quality Longjing rice seeds for 12 hours, wherein the weight ratio of the soaking s...
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