Culture for controlling turf grass root rot and fermentation method

Abstract

The invention belongs to the technical field of bacterial identification analysis and discloses a culture for controlling turf grass root rot and a fermentation method. The method comprises the following steps: separating and purifying bacteria by adopting a dilution-plate method, culturing on an NA test tube slant for 18-24 hours, and preserving in a refrigerator at 4 DEG C; calculating the antibacterial rate by adopting a fungus cake agar disk diffusion method, and preliminarily screening antagonistic strains having the inhibition ratio of 50% or higher to two and more than two root rot fungi; inoculating a strain HCST9 in an NB culture medium by adopting a fluid-culture method, adding equivalent NB culture medium into a control culture flask of a PS culture medium, weighing after drying, and calculating the bacteriostasis rate of the strain; and respectively calculating and measuring the bacteriostasis rate by adopting the fluid-culture method and the agar disk diffusion method. According to the method disclosed by the invention, turf grass root rot antagonistic bacteria are separated from turf soil from the biological control means, the bacteria strains with excellent antagonism are identified, the shaking flask fermentation conditions are optimized, and a theoretical basis is provided for large-scale industrial fermentation production in future.

Description

technical field [0001] The invention belongs to the technical field of bacterial identification and analysis, and in particular relates to a strain and a fermentation method for preventing and treating turfgrass root rot. Background technique [0002] Lawn has increasingly become an indispensable environmental landscape in people's life, but its disease damage is relatively serious. Lawn root rot is caused by a variety of pathogenic bacteria alone or in combination. The lesions mainly occur in the roots and rhizomes. The stems and leaves above the ground turn yellow in the early stage and wither in the later stage, which reduces the ornamental value of the lawn and causes lawn root rot. The pathogens of rot mainly include Fusarium sp., Rhizoctonia sp., Pythium sp., Biploarissp, etc. At the same time, Drechslera sp., Curuularia sp. etc. can also cause turf root rot symptoms. The prevention and control of turf diseases is different from other crops. While controlling turf di...

AI Technical Summary

Problems solved by technology

[0003] In summary, the problems in the prior art are: There is a lack of research on screening and transforming antagonistic strains in existing research inventions

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