Microbial detection method

Abstract

The invention discloses a microbial detection method, wherein the method comprises the following steps: an antibody-labeled magnetic bead microsphere is constructed, and a target microbial antibody tobe detected is combined with the magnetic bead microsphere to form a complex; a specific recognition target microorganism is prepared and can be combined with the target microorganism to form a complex with an exogenous nucleic acid, and the exogenous introduced nucleic acid sequence is not correlated to bacterial genome sequences; and then coupling is performed to form a microbial-antibody-magnetic bead complex. The specific nucleic acid sequence of the target microorganism is adsorbed by the complex, and then the obtained complex is desorbed, amplified and detected to obtain microbial detection results. The method can quickly and accurately detect various macromolecular microorganisms such as bacteria, viruses, pathogens, cells or proteins.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting microorganisms. Background technique [0002] The traditional microbial detection method is the culture method, which is still used by the national standard. Although this method is reliable and accurate, it often takes three days or even longer due to the need to enrich the bacteria before serological identification or other biochemical methods. In some perishable There is not such a long time to wait for detection on food or food that is out of customs. The development of a fast and convenient method for detecting microorganisms needs to be solved. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for detecting microorganisms that satisfies the requirements of simplicity, speed and low cost on the premise of realizing accurate detection. [0004] In order to solve the abov...

AI Technical Summary

Problems solved by technology

[0002] The traditional microbial detection method is the culture method, which is still used by the national standard. Although this method is reliable and accurate, it often takes three days or even longer due to the need to enrich the bacteria before serological identification or other biochemical methods. In some perishable There is not such a long time to wait for detection on food or food that has been exported from customs. The development of fast and convenient detection of microorganisms needs to be solved.