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Soft fat fixing liquid and preparation method thereof

A technique of fixative and soft fat, which is applied in the preparation, sampling, measuring device and other directions of test samples, can solve the problem of unsuitable fixed specimens, etc., achieve accurate positive rate of lymph node metastasis, increase the number of detections, and reduce the time for dissection and sampling Effect

Inactive Publication Date: 2018-12-07
SHAOXING PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problem with this method is that it is not applicable to fixed specimens that are more common in clinical practice.

Method used

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  • Soft fat fixing liquid and preparation method thereof
  • Soft fat fixing liquid and preparation method thereof
  • Soft fat fixing liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]Embodiment 1: the ointment fixative of different compositions

[0032] The core functional components of the palmitate fixative solution prepared by the present application are mainly formaldehyde, lecithin, absolute ethanol and acetone, and in different implementation environments, additional deoxycholic acid, methanol, PBS solution and any One or more, the specific composition selection method can refer to Table 1.

[0033] Table 1 different compositions of ointment fixative

[0034]

[0035] We tested the core components of the ointment fixative (i.e. No. 1 in Table 1) and supplementary components respectively, and the above 16 situations can be obtained. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: The soft lipid fixative of the core component has a good fixation effect on adipose tissue, organ tissue (such as thyroid tissue, breast tissue, lung tissue) or colon tissue, and the lymph nod...

Embodiment 2

[0038] Embodiment 2: the same composition different concentrations of ointment fixed solution

[0039] The present embodiment provides the composition and concentration of the palmitate fixative solution of the whole composition (i.e. including all core constituents and all supplementary constituents), and several representative groups are listed as shown in Table 2:

[0040] Table 2 different concentrations of palmitate fixative

[0041]

[0042]

[0043] The pretreated surgically resected omentum specimens were treated with the omentum fixative solution corresponding to the serial numbers in Table 2 for 4 hours, and then the lymph nodes were collected, dehydrated, embedded, sectioned, stained, immunohistochemical, and observed under a microscope. The results showed that: The above-mentioned concentrations can dissolve the sample to varying degrees, the fat is soft and liquefied, the lymph nodes are effectively exposed, the cell morphology of the lymph nodes is preserve...

Embodiment 3

[0044] Embodiment 3: Effect comparison of different preparation methods

[0045] During our experiments, we found that the different addition sequences of various raw materials (including core active ingredients such as and supplementary ingredients such as deoxycholic acid, methanol, PBS solution, and melanin) will also affect the effect of palmitate. As: According to the characteristic that ethanol is miscible with various organic solvents such as water, methanol, and ether in any proportion, firstly, methanol and acetone are miscible with ethanol, and then according to the hydrophilicity of lecithin, lecithin is dissolved in PBS (less than 50 degrees to avoid failure), then add water-soluble formaldehyde solution to PBS in sequence, and finally mix ethanol mixture and PBS mixture and stir evenly.

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Abstract

The invention relates to a soft fat fixing liquid and a preparation method thereof, and belongs to the technical field of sample preparation for testing. Anhydrous ethanol, lecithin, formaldehyde andacetone are used as raw materials, the anhydrous ethanol, the formaldehyde and the acetone are evenly stirred, the lecithin is added and stirred to dissolve, and the prepared soft fat fixing liquid comprises 50-150ml / L of the formaldehyde, 30-70g / L of the lecithin, 200-300ml / L of the anhydrous ethanol and 200-300ml / L of the acetone. The preparation method is used in preparation of the soft fat fixing liquid, especially in fixation of lymph node dissection for drawing materials in cancer samples, ensures timely and sufficient fixation of lymph nodes, reduces time of pathologist's dissection fordrawing the materials, and also ensures the integrity of lymph node cell morphology, lymph node cells have no deformation and have a good immunohistochemical effect, and the number of detected lymphnodes and accurate lymph node metastasis positive rate are effectively improved.

Description

technical field [0001] The application relates to a palmitate fixative and a preparation method thereof, belonging to the technical field of test sample preparation. Background technique [0002] The main way of cancer metastasis is the lymphatic channel. Cancer cells metastasize to lymph nodes through lymphatic drainage, which not only causes local tissue damage, but also hides in lymph nodes and becomes the origin of metastasis of other organs. Resection includes radical treatment of primary tumor and drainage regional lymph nodes Sexual surgery is the most important surgical treatment for cancer patients. Lymph node anatomy and pathological sampling after radical surgery for cancer is one of the important steps in clinical diagnosis and treatment. Studies have shown that adequate radical resection of lymph nodes can greatly reduce cancer-related events, and even if all lymph nodes have no metastasis in pathological examination, it has an important impact on the long-term...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/305
Inventor 刘芳张晓苹杨烨赵宏军陶锋孙爱静
Owner SHAOXING PEOPLES HOSPITAL
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