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Degradation strain of sterilization agent kresoxim-methyl and application thereof

A fungicide and strain technology, applied in the field of biodegradation, to achieve the effect of enriching the germplasm resource bank

Active Publication Date: 2018-12-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing technology for degradation and restoration of methoxyacrylate fungicides such as kresoxim-methyl and other methoxyacrylate fungicides, and to provide a new plant that degrades methoxyacrylate fungicides and pesticides such as kresosystrobin. The bacterial strain can quickly and efficiently degrade kresoxim-methyl, etc., and can be used to repair soil and water bodies and other environments polluted by kresoxim-methyl residues

Method used

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  • Degradation strain of sterilization agent kresoxim-methyl and application thereof
  • Degradation strain of sterilization agent kresoxim-methyl and application thereof
  • Degradation strain of sterilization agent kresoxim-methyl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Example 1 Isolation and Identification of Bacterial Strains

[0040] 1. Screening and isolation of kresoxim-methyl-degrading strains:

[0041] The activated sludge from the sewage outlet of a pesticide factory in Foshan, Guangdong was collected, and 5 g of the activated sludge sample was weighed and added to 50 mL of the above-mentioned MSM liquid medium containing kresoxstrobin (50 mg / L). After culturing at 30°C and 200 rpm for 7 days, the mass concentration of pesticides was increased from 50 mg / L to 100 mg / L, 200 mg / L, 400 mg / L, and 800 mg / L in sequence at 10% inoculum size each time. L continuous enrichment culture. Then, the culture medium transferred for 4 times was serially diluted and spread on the LB solid plate containing 50 mg / L kresoxim-methyl, and cultured upside down at 30°C for 2 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a high-efficiency bacterium, numbered TS-04.

[00...

Embodiment 2

[0058] Example 2 Degradation effect experiment of bacterial strain TS-04 on kresoxim-methyl

[0059] 1. Experimental method

[0060] (1) Seed solution preparation: Inject the purified strain TS-04 into 5 mL of LB liquid medium for overnight activation and culture to the logarithmic phase. After centrifugation at 4°C, the bacteria were washed with normal saline (0.9% NaCl) , and the resulting bacteria were used as inoculum.

[0061] (2) Degradation performance measurement: according to the inoculation amount of 0.3 g / L wet bacteria (equivalent to 1.0×10 7 CFU / mL), were inoculated into 50 mL of MSM culture solution containing kresoxim-methyl (25 mg / L), and no inoculation was used as the control, and each group had three repetitions. Incubate at 30°C, 200 rpm in a constant temperature shaker for 5 days, take samples every 1 day, and measure OD with a spectrophotometer 600 The value represents the growth of strain TS-04, and its degradation of kresoxim-methyl was determined by ...

Embodiment 3

[0068] Example 3 Research on the Degradation Characteristics of Kresstrobin by Strain TS-04

[0069] 1. Experimental method

[0070] (1) The effect of pH value on the degradation of kresoxim-methyl by TS-04:

[0071] Inoculate 50 mL of MSM culture solution with different pH values ​​(5.0, 6.0, 7.0, 8.0, 9.0) at an inoculum of 0.3 g / L wet bacteria (equivalent to 1.0×10 7 CFU / mL), and kresoxim-methyl was added to make the final concentration 25 mg / L, and no inoculation was used as the control, and each group had three repetitions. After culturing on a constant temperature shaker at 30 °C and 200 rpm for 4 days, the degradation of kresoxim-methyl by strain TS-04 under different pH conditions was determined by HPLC.

[0072] (2) Effect of temperature on the degradation of kresoxim-methyl by TS-04:

[0073] Inoculate 50 mL of MSM culture medium with an inoculum of 0.3 g / L wet bacteria (equivalent to 1.0×10 7 CFU / mL), and kresoxim-methyl was added to make the final concentrati...

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Abstract

The invention discloses a degradation strain of a sterilization agent kresoxim-methyl and application thereof. The strain is Sinomonas soli TS-04, is preserved in Guangdong province Center for Type Culture Collection on July 5th, 2018, and has the preservation number of GDMCC No:60410. The invention discloses the degradation effect of Sinomonas soli on Strobilurin type stelriztion agents such as kresoxim-methyl for the first time; the efficient and fast degradation strain TS-04 is obtained through screening; in addition, the bacterium can effectively degrade kresoxim-methyl in a wide pH and temperature range; the tolerance on high-concentration sterilization agent is realized; the strain can be used as a good pesticide bacterium to be applied to the aspects of biological restoration on polluted environment such as water bodies and soil. The invention provides a novel development path for breaking the existing pesticide residue pollution government bottleneck, enriches the genetic resources bank of the pesticide degradation bacterium and has good application prospects.

Description

Technical field [0001] The invention belongs to the technical field of biodegradation. More specifically, it relates to a degrading strain of the fungicide acetostrobin and its application. Background technique [0002] Common types of methoxyacrylate fungicides include azoxystrobin, azoxystrobin, and pyraclostrobin. They are currently the highest-selling protective fungicides in the world and have the longest duration among chemical protective fungicides. Element. This type of fungicide has good systemic activity and broad bactericidal spectrum. It has good control effect on fungal plant diseases such as strawberry powdery mildew, cucumber powdery mildew, pear scab, rice blast, etc. It has control effect on almost all fungal diseases. , effective against all fungal diseases of grapes. Therefore, it is widely used. However, the safety of different components of methoxyacrylate fungicides is quite different; in addition to its bactericidal activity, it also has a certain ...

Claims

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Application Information

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IPC IPC(8): A62D3/02C12N1/20C02F3/34B09C1/10A62D101/04A62D101/28C12R1/01C02F101/38
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 陈少华滕诗雨冯彦媚占卉顾雅雯郭毅博温小云
Owner SOUTH CHINA AGRI UNIV
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