Method for extracting proteins from peony seed meal
A technology of peony seed meal and protein, applied in the direction of fermentation, etc., can solve the problems of environmental impact, waste of resources, etc., achieve high extraction rate, promote development, and accelerate dissolution
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[0021] Example 1
[0022] Extract protein from peony seed meal
[0023] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C, and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. Store the obtained dry matter at a low temperature for later use
[0024] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:10, then add cellulase at 50°C and pH 5.0 for 0.5h. Obtain enzyme hydrolysate I; among them, the added amount of cellulase is 0.5% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U / g; The unit of material ratio is g:mL;
[0025] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 10, and then perform ultrasonic extraction for 90 minutes at a temperature of 50°C and a power of 160W;
[0026] (4) Obta...
Example Embodiment
[0029] Example 2
[0030] Extract protein from peony seed meal
[0031] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;
[0032] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water according to the material ratio of 1:12, then add cellulase at 45°C and pH value of 5.5 for enzymatic hydrolysis for 0.25h, Obtain enzymatic hydrolysis solution I; among them, the added amount of cellulase is 0.8% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U / g; The unit of material ratio is g:mL;
[0033] (3) Ultrasonic alkaline extraction: adjust the pH value of enzymatic hydrolysis solution I in step (2) to 8, and then perform ultrasonic extraction for 70 minutes a...
Example Embodiment
[0037] Example 3
[0038] Extract protein from peony seed meal
[0039] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;
[0040] (2) Enzymatic hydrolysis: Add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:8, then add cellulase at 55°C and pH 4.0, and enzymatically hydrolyze for 1 hour to obtain Enzymatic hydrolysis solution I; the amount of cellulase added is 0.2% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U / g; The unit of material ratio is g:mL;
[0041] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 12, and then perform ultrasonic extraction for 110 minutes at a temperature of 30°C and...
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