Method for extracting proteins from peony seed meal

A technology of peony seed meal and protein, applied in the direction of fermentation, etc., can solve the problems of environmental impact, waste of resources, etc., achieve high extraction rate, promote development, and accelerate dissolution

Inactive Publication Date: 2018-12-14

SOUTHWEST UNIVERSITY

5 Cites 5 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0002] Peony seed meal is the main by-product of peony seed oil extraction, but because peony seed meal has not been effectively used, most of it is used as feed or directly discarded, which not only causes a lot of waste of resources, but also has a certain impact on the environment

Peony seed mea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Abstract

The invention relates to a method for extracting proteins from peony seed meal, and belongs to the technical field of plant extraction. The method comprises six steps: degreasing, enzymatic hydrolysis, ultrasonic alkali extraction, obtaining of a crude peony seed meal protein, purification and obtaining of the peony seed meal protein. Enzymatic hydrolysis and ultrasonic alkali extraction are cooperatively used for extracting the protein from the peony seed meal, and various process conditions in the extraction process are set reasonably to ensure high protein extraction rate. The method is simple and easy to operate, low in cost and suitable for industrialized production and promotes the development of peony seed oil and deep processing industrialization.

Application Domain

Fermentation

Technology Topic

Protein proteinEnzymatic hydrolysis +4

Examples

Experimental program(3)

Example Embodiment

[0021] Example 1

[0022] Extract protein from peony seed meal

[0023] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C, and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. Store the obtained dry matter at a low temperature for later use

[0024] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:10, then add cellulase at 50°C and pH 5.0 for 0.5h. Obtain enzyme hydrolysate I; among them, the added amount of cellulase is 0.5% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;

[0025] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 10, and then perform ultrasonic extraction for 90 minutes at a temperature of 50°C and a power of 160W;

[0026] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 4000 r/min for 20 minutes to obtain the supernatant, adjust the pH of the supernatant to 4.5, and then again Centrifuge at 4000r/min for 20 minutes to obtain Precipitate I. After washing the Precipitate I to neutrality, freeze-dry it in a freeze dryer for 15 hours to obtain crude protein of peony seed meal;

[0027] (5) Purification: Add the crude protein of peony seed meal obtained in step (4) to distilled water according to the material ratio of 1:10, then add glucoamylase at 50°C and pH value of 3.5, and enzymatically hydrolyze for 20 minutes to obtain the enzyme Solution II; the added amount of glucoamylase is 0.02% of the crude protein quality of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;

[0028] (6) Obtain peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at 4000r/min for 20 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 15 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 91.43%.

Example Embodiment

[0029] Example 2

[0030] Extract protein from peony seed meal

[0031] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;

[0032] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water according to the material ratio of 1:12, then add cellulase at 45°C and pH value of 5.5 for enzymatic hydrolysis for 0.25h, Obtain enzymatic hydrolysis solution I; among them, the added amount of cellulase is 0.8% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;

[0033] (3) Ultrasonic alkaline extraction: adjust the pH value of enzymatic hydrolysis solution I in step (2) to 8, and then perform ultrasonic extraction for 70 minutes at a temperature of 70°C and a power of 180W;

[0034] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 3500r/min for 30min to obtain the supernatant, adjust the pH of the supernatant to 5.0 and then again Centrifuge at a speed of 3500r/min for 30min to obtain Precipitate I. After washing the Precipitate I to neutrality, freeze-dry it in a freeze dryer for 16h to obtain crude protein of peony seed meal;

[0035] (5) Purification: Add the crude protein of peony seed meal obtained in step (4) to distilled water at a material ratio of 1:12, then add glucoamylase at 60°C and pH value of 5, and enzymatically hydrolyze for 10 minutes to obtain the enzyme Solution Ⅱ; the added amount of glucoamylase is 0.05% of the crude protein of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;

[0036] (6) Obtain peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at a speed of 3500r/min for 30 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 16 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 90.8%.

Example Embodiment

[0037] Example 3

[0038] Extract protein from peony seed meal

[0039] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;

[0040] (2) Enzymatic hydrolysis: Add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:8, then add cellulase at 55°C and pH 4.0, and enzymatically hydrolyze for 1 hour to obtain Enzymatic hydrolysis solution I; the amount of cellulase added is 0.2% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;

[0041] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 12, and then perform ultrasonic extraction for 110 minutes at a temperature of 30°C and a power of 140W;

[0042] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 5000r/min for 15min to obtain the supernatant, adjust the pH of the supernatant to 4.0 and then again Centrifuge at 5000r/min for 15min to obtain Precipitate I. After washing Precipitate I to neutrality, freeze-dried for 14h in a freeze dryer to obtain crude protein of peony seed meal;

[0043] (5) Purification: add the crude protein of peony seed meal obtained in step (4) to distilled water according to the material ratio of 1:8, then add glucoamylase at 40°C and pH 3, and enzymatically hydrolyze for 50 minutes to obtain the enzyme Solution II; the added amount of glucoamylase is 0.01% of the crude protein of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;

[0044] (6) Obtaining peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at 5000r/min for 15 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 14 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 89.66%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

Classification and recommendation of technical efficacy words

High extraction rate
Promote dissolution

People also interested in

Low cost Improve development Meal

Method for extracting proteins from peony seed meal

A technology of peony seed meal and protein, applied in the direction of fermentation, etc., can solve the problems of environmental impact, waste of resources, etc., achieve high extraction rate, promote development, and accelerate dissolution

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Method for purifying and separating phenolic compounds in magnolia medicament

Method for preparing kaempferol

Production method for extracting lignite wax from brown coal

Method for extracting and purifying flavonoids of artemisia argyi

Enzymatic pretreatment assisted curcumin extraction technology

Classification and recommendation of technical efficacy words

Method for preparing sea-tangle polysaccharide

Method for decomposing vanadium slag under normal pressure with sodium hydroxide solution

Chinese medicinal herb-seaweed composite biological agent and preparation method thereof

Method for continuously generating fine gutta-percha through digestion-reextraction

Method for extracting proteins and chitin from heads and shells of prawns

Bilayer tablet comprising telmisartan and amlodipine

Limestone-gypsum wet desulfurization additive

Method for extracting toosendanin from cortex meliae

Dispersible tablet of maca, preparation method and application thereof

Amoxicillin lipidosome solid preparation and new application thereof

People also interested in