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Method for extracting proteins from peony seed meal

A technology of peony seed meal and protein, applied in the direction of fermentation, etc., can solve the problems of environmental impact, waste of resources, etc., achieve high extraction rate, promote development, and accelerate dissolution

Inactive Publication Date: 2018-12-14
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Peony seed meal is the main by-product of peony seed oil extraction, but because peony seed meal has not been effectively used, most of it is used as feed or directly discarded, which not only causes a lot of waste of resources, but also has a certain impact on the environment
Peony seed meal contains high protein, and the application of protein in food is very promising, but there are few reports about peony protein. Experiments have proved that peony protein has the functions of lowering cholesterol, anti-oxidation, and improving cardiovascular diseases. beneficial effect on health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Extraction of protein from peony seed meal

[0023] (1) Degreasing: crush the raw peony seed meal and pass it through an 80-mesh sieve, then use Soxhlet extraction to degrease, the degreasing temperature is 55°C, and the degreasing time is 6h. After the degreasing is completed, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for subsequent use;

[0024] (2) Enzymolysis: add the peony seed meal treated in step (1) into distilled water according to the material ratio of 1:10, then add cellulase at 50°C and pH value of 5.0, and perform enzymolysis for 0.5h. Enzyme hydrolyzate I was obtained; wherein, the amount of cellulase added was 0.5% of the mass of peony seed meal, and the enzyme activity was 1×10 4 U / g; the unit of material ratio is g:mL;

[0025] (3) Ultrasonic alkaline extraction: adjust the pH value of the enzymatic solution I in step (2) to 10, and then perform ultrasonic extraction for 90 minutes at a tempe...

Embodiment 2

[0030] Extraction of protein from peony seed meal

[0031] (1) Degreasing: crush the raw peony seed meal and pass it through an 80-mesh sieve, then use Soxhlet extraction to degrease, the degreasing temperature is 55°C, and the degreasing time is 6h. After the degreasing is completed, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for subsequent use;

[0032] (2) Enzymolysis: add the peony seed meal treated in step (1) into distilled water according to the material ratio of 1:12, then add cellulase at 45°C and pH value of 5.5, and perform enzymolysis for 0.25h. Enzyme hydrolyzate I was obtained; wherein, the added amount of cellulase was 0.8% of the mass of peony seed meal, and the enzyme activity was 1×10 4 U / g; the unit of material ratio is g:mL;

[0033] (3) Ultrasonic alkaline extraction: adjust the pH value of the enzymatic solution I in step (2) to 8, and then perform ultrasonic extraction for 70 minutes at a tempe...

Embodiment 3

[0038] Extraction of protein from peony seed meal

[0039] (1) Degreasing: crush the raw peony seed meal and pass it through an 80-mesh sieve, then use Soxhlet extraction to degrease, the degreasing temperature is 55°C, and the degreasing time is 6h. After the degreasing is completed, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for subsequent use;

[0040] (2) Enzymolysis: add the peony seed meal treated in step (1) into distilled water according to the material ratio of 1:8, then add cellulase at 55°C and pH value of 4.0, and perform enzymolysis for 1 hour to obtain Enzyme hydrolysis solution Ⅰ; wherein, the added amount of cellulase is 0.2% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U / g; the unit of material ratio is g:mL;

[0041] (3) Ultrasonic alkaline extraction: adjust the pH value of the enzymatic solution I in step (2) to 12, and then perform ultrasonic extraction for 110 min at a tempe...

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Abstract

The invention relates to a method for extracting proteins from peony seed meal, and belongs to the technical field of plant extraction. The method comprises six steps: degreasing, enzymatic hydrolysis, ultrasonic alkali extraction, obtaining of a crude peony seed meal protein, purification and obtaining of the peony seed meal protein. Enzymatic hydrolysis and ultrasonic alkali extraction are cooperatively used for extracting the protein from the peony seed meal, and various process conditions in the extraction process are set reasonably to ensure high protein extraction rate. The method is simple and easy to operate, low in cost and suitable for industrialized production and promotes the development of peony seed oil and deep processing industrialization.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting protein in peony seed dregs. Background technique [0002] Peony seed meal is the main by-product of peony seed oil extraction, but because peony seed meal has not been effectively utilized, most of it is used as feed or directly discarded, which not only causes a lot of waste of resources, but also has a certain impact on the environment. Peony seed meal contains high protein, and the application of protein in food is very promising, but there are few reports about peony protein. Experiments have proved that peony protein has the functions of lowering cholesterol, anti-oxidation, and improving cardiovascular diseases. Healthy and beneficial effects. The yield of mature peony seeds is 300kg-500kg / year. After the Ministry of Health approved peony seed oil as a new resource food in 2011, the industrialization of peony seed oil and its d...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张玉
Owner SOUTHWEST UNIVERSITY
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