Method for extracting proteins from peony seed meal

A technology of peony seed meal and protein, applied in the direction of fermentation, etc., can solve the problems of environmental impact, waste of resources, etc., achieve high extraction rate, promote development, and accelerate dissolution

Inactive Publication Date: 2018-12-14
SOUTHWEST UNIVERSITY
5 Cites 5 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0002] Peony seed meal is the main by-product of peony seed oil extraction, but because peony seed meal has not been effectively used, most of it is used as feed or directly discarded, which not only causes a lot of waste of resources, but also has a certain impact on the environment
Peony seed mea...
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Abstract

The invention relates to a method for extracting proteins from peony seed meal, and belongs to the technical field of plant extraction. The method comprises six steps: degreasing, enzymatic hydrolysis, ultrasonic alkali extraction, obtaining of a crude peony seed meal protein, purification and obtaining of the peony seed meal protein. Enzymatic hydrolysis and ultrasonic alkali extraction are cooperatively used for extracting the protein from the peony seed meal, and various process conditions in the extraction process are set reasonably to ensure high protein extraction rate. The method is simple and easy to operate, low in cost and suitable for industrialized production and promotes the development of peony seed oil and deep processing industrialization.

Application Domain

Technology Topic

Examples

  • Experimental program(3)

Example Embodiment

[0021] Example 1
[0022] Extract protein from peony seed meal
[0023] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C, and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. Store the obtained dry matter at a low temperature for later use
[0024] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:10, then add cellulase at 50°C and pH 5.0 for 0.5h. Obtain enzyme hydrolysate I; among them, the added amount of cellulase is 0.5% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;
[0025] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 10, and then perform ultrasonic extraction for 90 minutes at a temperature of 50°C and a power of 160W;
[0026] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 4000 r/min for 20 minutes to obtain the supernatant, adjust the pH of the supernatant to 4.5, and then again Centrifuge at 4000r/min for 20 minutes to obtain Precipitate I. After washing the Precipitate I to neutrality, freeze-dry it in a freeze dryer for 15 hours to obtain crude protein of peony seed meal;
[0027] (5) Purification: Add the crude protein of peony seed meal obtained in step (4) to distilled water according to the material ratio of 1:10, then add glucoamylase at 50°C and pH value of 3.5, and enzymatically hydrolyze for 20 minutes to obtain the enzyme Solution II; the added amount of glucoamylase is 0.02% of the crude protein quality of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;
[0028] (6) Obtain peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at 4000r/min for 20 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 15 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 91.43%.

Example Embodiment

[0029] Example 2
[0030] Extract protein from peony seed meal
[0031] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;
[0032] (2) Enzymatic hydrolysis: add the peony seed meal treated in step (1) to distilled water according to the material ratio of 1:12, then add cellulase at 45°C and pH value of 5.5 for enzymatic hydrolysis for 0.25h, Obtain enzymatic hydrolysis solution I; among them, the added amount of cellulase is 0.8% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;
[0033] (3) Ultrasonic alkaline extraction: adjust the pH value of enzymatic hydrolysis solution I in step (2) to 8, and then perform ultrasonic extraction for 70 minutes at a temperature of 70°C and a power of 180W;
[0034] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 3500r/min for 30min to obtain the supernatant, adjust the pH of the supernatant to 5.0 and then again Centrifuge at a speed of 3500r/min for 30min to obtain Precipitate I. After washing the Precipitate I to neutrality, freeze-dry it in a freeze dryer for 16h to obtain crude protein of peony seed meal;
[0035] (5) Purification: Add the crude protein of peony seed meal obtained in step (4) to distilled water at a material ratio of 1:12, then add glucoamylase at 60°C and pH value of 5, and enzymatically hydrolyze for 10 minutes to obtain the enzyme Solution Ⅱ; the added amount of glucoamylase is 0.05% of the crude protein of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;
[0036] (6) Obtain peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at a speed of 3500r/min for 30 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 16 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 90.8%.

Example Embodiment

[0037] Example 3
[0038] Extract protein from peony seed meal
[0039] (1) Degreasing: The raw peony seed meal is crushed and passed through an 80-mesh sieve, and then defatted by Soxlet extraction. The degreasing temperature is 55°C and the degreasing time is 6 hours. After degreasing, place a fume hood to volatilize petroleum ether. The obtained dry matter is stored at low temperature for later use;
[0040] (2) Enzymatic hydrolysis: Add the peony seed meal treated in step (1) to distilled water at a material ratio of 1:8, then add cellulase at 55°C and pH 4.0, and enzymatically hydrolyze for 1 hour to obtain Enzymatic hydrolysis solution I; the amount of cellulase added is 0.2% of the mass of peony seed meal, and the enzyme activity is 1×10 4 U/g; The unit of material ratio is g:mL;
[0041] (3) Ultrasonic alkaline extraction: adjust the pH of the enzymatic hydrolysis solution I in step (2) to 12, and then perform ultrasonic extraction for 110 minutes at a temperature of 30°C and a power of 140W;
[0042] (4) Obtain the crude protein of peony seed meal: Centrifuge the enzymatic hydrolysate I treated in step (3) at 5000r/min for 15min to obtain the supernatant, adjust the pH of the supernatant to 4.0 and then again Centrifuge at 5000r/min for 15min to obtain Precipitate I. After washing Precipitate I to neutrality, freeze-dried for 14h in a freeze dryer to obtain crude protein of peony seed meal;
[0043] (5) Purification: add the crude protein of peony seed meal obtained in step (4) to distilled water according to the material ratio of 1:8, then add glucoamylase at 40°C and pH 3, and enzymatically hydrolyze for 50 minutes to obtain the enzyme Solution II; the added amount of glucoamylase is 0.01% of the crude protein of peony seed meal, and the enzyme activity is 1×10 5 U/g; The unit of material ratio is g:mL;
[0044] (6) Obtaining peony seed meal protein: Centrifuge the enzyme hydrolysate II obtained in step (5) at 5000r/min for 15 minutes to obtain precipitation II, wash the precipitate II to neutrality and freeze-dry it with a freeze dryer for 14 hours , The peony seed meal protein is obtained, and the purity of the peony seed meal protein is 89.66%.
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