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Screening method for new tumor antigen

An antigen and tumor technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of time-consuming, high-cost, labor-intensive, etc., and achieve the effect of reducing the number of experiments, saving money, and saving labor and expenses.

Active Publication Date: 2018-12-18
BETA PHARM SUZHOU LTD
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Problems solved by technology

[0004] However, the development of tumor vaccines is currently screened one by one by means of experiments. This process is time-consuming, laborious, and expensive, and it is difficult to find suitable tumor vaccines (tumor antigens). The method of screening tumor neoantigens is to obtain a suitable tumor vaccine by screening tumor neoantigens, so as to facilitate subsequent further targeted experiments based on this, so as to greatly reduce the number of experiments, and achieve time-saving, labor-saving and cost-saving

Method used

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  • Screening method for new tumor antigen
  • Screening method for new tumor antigen

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Embodiment 1

[0042] Such as figure 1 As stated, this embodiment provides a method for screening tumor neoantigens, comprising the steps of:

[0043] S101. Obtain the polypeptide sequence encoded by the mutant gene of the patient's tumor tissue cells. Specifically, including:

[0044] A. Extract the DNA of tumor tissue cells by SDS method, and perform DNA sequencing on it;

[0045]B. Comparing the sequenced DNA sequence with the normal wild-type DNA sequence of the tissue cell to obtain a mutated DNA sequence different from the normal wild-type DNA sequence of the tissue cell. Wherein, the normal DNA sequence of the tissue cells may be acquired through an existing database. Wherein, the database may be: COSMIC, NCBI, UCSC, Ensembl, TCGA, etc.

[0046] C. Obtain the polypeptide sequence encoded by the mutated DNA sequence through biological software. Wherein, the biological software can be DNA-man, or other software that can translate DNA sequences into amino acid sequences. Wherein, t...

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Abstract

The invention provides a screening method for a tumor new antigen, and the method comprises: A, obtaining polypeptide sequences encoded corresponding to mutant genes of a tumor tissue cell; B, performing predicting on the affinity of each polypeptide sequence with a major histocompatibility complex MHCI, and screening a polypeptide sequence having the affinity exceeding a specified threshold; andC, predicting polypeptide enzyme digestion sites of the polypeptide sequences encoded corresponding to the mutant genes, and retaining the polypeptide sequences in which the enzyme digestion sites arenot in the polypeptide sequence having the affinity exceeding the specified threshold and which can be separated into segments and cannot be enzyme-digested, as candidate polypeptide sequences of thetumor new antigen. The screening method for the tumor new antigen facilitates subsequent further targeted experiments based on the screening for the tumor new antigen, can greatly reduce the number of the experiments, and saves time, labors and expenditures.

Description

technical field [0001] The invention relates to the field of antigen screening, in particular to a method for screening tumor neoantigens. Background technique [0002] Tumor vaccine is one of the research hotspots in recent years. Its principle is to introduce tumor antigens into patients in various forms, such as tumor cells, tumor-associated proteins or polypeptides, and genes expressing tumor antigens, so as to overcome the immune system caused by tumors. Suppressive state, enhance immunogenicity, activate the patient's own immune system, induce the body's cellular and humoral immune responses, so as to achieve the purpose of controlling or eliminating tumors. In April 2010, the US Food and Drug Administration (FDA) approved Provenge / sipuleucel-T for the treatment of advanced prostate cancer, making it the first autologous active immunotherapy drug and the first real therapeutic cancer vaccine. Paving the way for the development of other similar products (1,2). [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/00
CPCC07K1/00
Inventor 张崇骞赵永浩马赛闫成海张晓霞彭继荣张晓东
Owner BETA PHARM SUZHOU LTD
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