High-efficiency and large-volume bottom magnetic enrichment device

A large-volume, high-efficiency technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve problems such as unsatisfactory collection efficiency of aerosols, unfavorable rotating liquid effective wrapping, inconvenient collection of liquid, etc. , to achieve the effect of shortening the enrichment time, increasing the volume and good enrichment efficiency

Pending Publication Date: 2018-12-18
杭州富集生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, since the shape of the sampling cup determines the agitation and mixing of the airflow during the aerosol collection process, and the effective mixing process of gas and liquid is the key step for the collection of aerosol particles into the liquid phase sample, the conical or Although the straight cylindrical structure can fully agitate the liquid in the upper part of the cup, the liquid at the bottom cannot be sufficiently agitated. At the same time, the consistent cup-shaped design at the bottom of the mouth is not conducive to the effective wrapping of the airflow by the rotating liquid, so that some gas samples are not subjected to gas-liquid Mixing leaves the liquid phase, which results in suboptimal efficiency of aerosol collection into the liquid phase
On the other hand, when collecting the sample to be tested in the liquid phase magnetic bead enrichment stage, the old sampling cup can only be adsorbed by the side wall due to the limitation of the sampling cup structure. The gravity of the magnetic beads itself affects the adsorption effect of the magnet, resulting in a long adsorption time. , the disadvantages of inconvenient magnetic enrichment liquid phase samples that cannot be fully poured into the collection liquid

Method used

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  • High-efficiency and large-volume bottom magnetic enrichment device
  • High-efficiency and large-volume bottom magnetic enrichment device
  • High-efficiency and large-volume bottom magnetic enrichment device

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037] Example 1 Evaluation of Enrichment Support Magnetic Beads Enrichment Effect

[0038] The old and new sampling cups were used for the magnetic bead enrichment test. First, add an appropriate amount of collection solution to the sampling cup, and then add three drops of magnetic beads (about 150 μl) in the same amount, and stir well to disperse the magnetic beads evenly in the collection solution. Put the sampling cup into the enrichment rack at the same time, and stop the timer. Repeat three times to record the time when the magnetic beads are completely enriched and the collected solution recovers to clear.

[0039] After repeated experiments, the average enrichment time of the bottom enrichment support was 3m24s, and the average enrichment time of the old enrichment support was 5m40s, and the enrichment time was shortened by 40%.

example 2

[0040]Example 2 Evaluation of the recovery effect of the new and old enrichment scaffold magnetic beads

[0041] The old and new sampling cups were used to carry out the magnetic bead recovery comparison experiment. After adding an appropriate amount of collection solution to each sampling cup, add three drops (about 150 μl) of magnetic beads in an equal amount, stir well to mix the magnetic beads evenly, and then carry out enrichment. After the magnetic beads are fully enriched and the collected solution is clarified, rotate the enrichment rack and pour the collected solution to obtain the enriched magnetic beads.

[0042] During the experiment, it was obvious that a small part of the magnetic beads flowed out with the poured liquid in the old-type enrichment scaffold, which caused the loss of biological samples. However, there is no visible loss of magnetic beads in the process of pouring the collection solution at the bottom of the enrichment bracket, which greatly avoids t...

example 3

[0043] Example 3 The change experiment of the magnet angle at the bottom of the bottom enrichment support

[0044] Six bottom magnet tilt angles of 110°, 120°, 130°, 140°, 150°, and 160° were designed for the bottom sampling bracket, and the magnetic bead enrichment time evaluation experiment was carried out on them.

[0045] The experiments were repeated three times for each of the six enrichment scaffolds, and the average enrichment time of the six enrichment scaffolds corresponded as follows:

[0046] Bracket angle(°)

[0047] Experiments show that except the enrichment time of 110° and 160° enrichment scaffolds is longer than that of the old-type enrichment scaffolds, the enrichment efficiency of the other four angles of enrichment scaffolds is better than that of the old-type enrichment scaffolds. Therefore, the enrichment bracket at the bottom has good enrichment efficiency within the range of the magnet angle of 120°-150°.

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Abstract

The invention provides a high-efficiency and large-volume bottom magnetic enrichment device, which comprises a frame, a gas supply device, an outlet unit nozzle, a sampling cup and a bottom enrichmentunit frame. The gas supply device inputs a gas sample into the outlet unit nozzle through a gas pipe. The outlet unit nozzle, the sampling cup and the bottom enrichment unit frame are all disposed onthe frame; the sampling cup is detachably disposed below the outlet unit nozzle; the bottom of the sampling cup is located on the bottom enrichment unit frame and they are in surface-to-surface contact; and a magnetic device is disposed at the contact surface between the bottom enrichment unit frame and the bottom of the sampling cup. The invention has the following beneficial effects: the methodof enriching the liquid-phase magnetic bead sample by the bottom magnetic force is shortened, the collecting thread or buckle is effectively fixed to the bottom surface of a collecting unit, the shortest operating distance between the magnet and the magnetic bead is guaranteed, and the magnetic contact surface is expanded, the efficiency of enriching magnetic beads is improved and the enrichmenttime is shortened.

Description

technical field [0001] The invention relates to the field of biological aerosol collection and enrichment, and more specifically relates to a high-efficiency large-volume bottom magnetic enrichment device. Background technique [0002] The Bio-capturer aerosol collection and enrichment instrument is a sampling instrument that enriches biological aerosol particles in real time through gas-liquid mixing and integrating liquid-phase magnetic beads. Hereinafter referred to as "sampling cup") initially adopted the centrifuge tube sampling design commonly used by commercial air samplers on the market. Note: the gas-liquid mixing sampling cups involved in commercial air microbial samplers on the market are generally straight ( Such as SKC BIO-Sampler, domestic AGI series air microbial sampler, etc.) or a narrow tapered bottom structure similar to the shape of a centrifuge tube or test tube (such as Coriolis air sampler, etc.). In actual research, we found that the gas-liquid impac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/28
CPCC12M33/00
Inventor 胡孔新李金珂马雪征李丽丽赵欣张丽萍
Owner 杭州富集生物科技有限公司
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