Kit for detecting porcine reproductive and respiratory syndrome virus and application thereof

Abstract

The invention discloses a kit for detecting porcine reproductive and respiratory syndrome virus. The kit comprises a PCR reaction buffer solution 1, a primer probe mixture 2,an enzyme system 3, and negative control and positive control, and the primer probe mixture 2 is composed of a pair of primers and a specific fluorescent probe. The primer sequence comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ IDNO: 3 and SEQ ID NO: 4, and the sequence of the fluorescent probe comprises SEQ ID NO: 5 and SEQ ID NO: 6. The invention has the following beneficial effects: the multiplex RT-PCR detection kit for the porcine reproductive and respiratory syndrome virus provided by the invention is fast compared with classical virus isolation, single RT-PCR and serum typing methods, and can identify American and European porcine reproductive and respiratory syndrome viruses in one time so as to facilitate timely and fast responses to epidemic disease situations, regions and environments, and correct detectionresult can be obtained in shortest time.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a kit and a detection method for detecting porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (Procine Reproductive and Respiratory Syndrome, PRRS) (also known as porcine blue ear disease) is caused by porcine reproductive and respiratory syndrome virus (Procine Reproductive and Respiratory Syndrome Virus, PRRSV). Highly contagious infectious disease characterized by premature birth, abortion, stillbirth, mummified fetus and piglet respiratory syndrome. According to different genotypes, the disease is divided into American type and European type. According to different clinical manifestations, PRRS can be divided into classic PRRS and highly pathogenic PRRS. Highly pathogenic PRRS is characterized by high contact transmission, systemic hemorrhage, lung consolidation and reproductive failur...

AI Technical Summary

Problems solved by technology

However, the phenomenon of immune infection and environmental infection is still very common. Due to the lack of necessary biosecurity measures in some small and medium-sized pig farms and scattered farmers, the epidemic still occurs from time to time

In addition, due to improper handling of pigs after the disease, it will also cause high mortality in the diseased pigs

[0005] At present, traditional PRRSV detection methods mainly include virus isolation and identification, immunoperoxidase monolayer assay (IPMA), indirect immunofluorescence assay (IFA), indirect enzyme-linked immunosorbent assay (indirect ELISA) and reverse transcription- Polymerase chain reaction test (RT-PCR), etc. These methods have played an important role in disease diagnosis and import and export inspection and quarantine, but each has insufficient sensitivity, specificity or timeliness, and is not suitable for rapid detection of diseases. Diagnosis, it is easy to miss the best time for epidemic prevention and control

[0006] In my country, the coexistence of various strains such as American classic PRRSV, mutant PRRSV, NADC30-like PRRSV, European PRRSV and vaccine strains has increased the difficulty of clinical diagnosis, and the prevention and control of PRRSV is facing unprecedented severe challenges

Images