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Staining liquid for flow analysis

A technology of flow analysis and staining solution, which is used in the preparation and sampling of analytical materials, samples for testing, etc., can solve the problems of reducing and increasing the number of blood cells, and achieves easy cell binding, stable homogeneous phase system, and accurate analysis results. Effect

Active Publication Date: 2018-12-18
武汉百合龙腾生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, certain blood cell counts increase or decrease when the subject has a disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 of the present invention provides a dyeing solution for flow analysis, including 100 parts of hydroxyl compounds, 0.01 parts of modified benzyl chloride and 0.01 parts of olefin derivatives;

[0039] The hydroxyl compound is CH 3 OH and HOCH 2 CH 2 OH; the CH 3 OH and HOCH 2 CH 2 The weight ratio of OH is 1:49;

[0040] The raw materials for the preparation of the modified benzyl chloride include 4-aminodiphenylamine and benzyl chloride; the molar ratio of the benzyl chloride to 2,4-diaminodiphenylamine is 1:1.2;

[0041] The preparation method of the modified benzyl chloride is as follows: under a nitrogen atmosphere, weigh benzyl chloride, 4-aminodiphenylamine and triethylamine in a 50mL reaction bottle, and then add N,N-dimethylformamide , stirred and reacted at 65°C for 5 hours, after the reaction was completed, washed three times with anhydrous ether, and dried to obtain the modified benzyl chloride;

[0042] The mol ratio of described triethyla...

Embodiment 2

[0046] Example 2 of the present invention provides a dyeing solution for flow analysis, the specific implementation of which is the same as that of Example 1, except that it includes 150 parts of hydroxyl compounds, 1 part of modified benzyl chloride and 1 part of olefin derivatives;

[0047] The CH 3 OH and HOCH 2 CH 2 The weight ratio of OH is 1:50;

[0048] The molar ratio of benzyl chloride to 2,4-diaminodiphenylamine is 1:1.5.

Embodiment 3

[0050] Example 3 of the present invention provides a dyeing solution for flow analysis, the specific implementation of which is the same as that of Example 1, except that it includes 60 parts of hydroxyl compounds, 0.001 parts of modified benzyl chloride and 0.001 parts of olefin derivatives;

[0051] The CH 3 OH and HOCH 2 CH 2 The weight ratio of OH is 1:30;

[0052] The molar ratio of benzyl chloride to 2,4-diaminodiphenylamine is 1:0.8.

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PUM

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Abstract

The invention relates to the field of the biological reagent, more specifically, the invention relates to staining liquid for flow analysis and a preparation method thereof. In the first part, the staining liquid for flow analysis is provided, the staining liquid is prepared from the following raw materials in part by weight: 60-150 parts of hydroxyl compound, 0.001-1 part of modified benzyl chloride and 0.001-1 part of olefin derivative; the hydroxyl compound is R1(CH2)nOH, R1 is methyl or hydroxyl, and n is 0-5 integer; the structure of the olefin derivative is selected from one or combination of multiple of the chemical formula as shown in description, wherein X is one of Br, I, ClO4, M is Na or K, and R1, R2 and R3 are respectively selected from one or multiple of hydrogen atom, hydrocarbonyl, acyl, aryl, cycloalkyl, and carboxyl.

Description

technical field [0001] The invention relates to the field of biological reagents, more specifically, the invention relates to a staining solution for flow analysis and a preparation method thereof. Background technique [0002] Flow cytometry (Flow Cytometry, FCM) is a new type of analysis and sorting technology that can quickly measure cells or subcellular structures. Its measurement speed is fast, and tens of thousands of cells can be detected within 1 second; Multi-parameter measurement, multi-parameter measurement of physical, chemical, biological and other characteristics of the same cell, and has obvious statistical significance. It integrates the knowledge and achievements in many fields such as laser technology, computer technology, fluid mechanics, cytochemistry, and image technology; it is not only a cell analysis technology, but also an accurate sorting technology. [0003] At present, flow cytometry has been widely used in various fields. Among them, the flow c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 王小海
Owner 武汉百合龙腾生物科技有限责任公司