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Optimized volumetric imaging with selective volume illumination and light field detection

A technology of illuminating light and volume, applied in optics, optical components, image analysis, etc., can solve the problems of reducing image contrast and accuracy, and achieve the effect of reducing possibility, high contrast and accuracy, and reducing background signal

Pending Publication Date: 2018-12-21
UNIV OF SOUTHERN CALIFORNIA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Thus, widefield illumination introduces extraneous background and noise, potentially reducing the contrast and accuracy of the final reconstructed image

Method used

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  • Optimized volumetric imaging with selective volume illumination and light field detection
  • Optimized volumetric imaging with selective volume illumination and light field detection
  • Optimized volumetric imaging with selective volume illumination and light field detection

Examples

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Embodiment Construction

[0018] figure 1 An example of a volume imaging system 100 employed to provide selective volume illumination (SVI) using light field detection is shown. For example, volumetric imaging system 100 provides light field capabilities, such as supporting 3D reconstruction of microscope sample 126 . According to an embodiment, the volumetric imaging system 100 may be employed to provide 3D segmentation and 3D imaging at cellular or better resolution at speeds at which dynamic processes can be captured (eg, low resolution / high speed volumetric). Such as figure 1 As shown, the system integrates various hardware, software, and firmware components that operate in concert to implement the selective volume illumination technique of an embodiment to illuminate 3D confined illumination of a sample 126 that has been determined to be related to a volume Imaging a specific portion of a 3D sample that is substantially correlated. That is, 3D limited illumination is the selected volume of the ...

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Abstract

Systems and techniques relating to optimizing volumetric imaging with selective volume illumination (SVI) using light field detection, in one aspect, include: a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample, wherein the light field propagates via a detection light path; wherein the light source, the optical system, or both, are configurable to select a volume ofa 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit under 35 U.S.C. §119(e) of U.S. Patent Application No. 62 / 300,469, filed February 26, 2016, entitled "OPTIMIZED VOLUMETRIC IMAGING WITHSELECTIVE VOLUME ILLUMINAYION AND LIGHT FIELD DETECTION," incorporated by reference It is incorporated herein in its entirety. Background technique [0003] This specification relates to the optimization of volumetric imaging using light field detection using a biomolecular imaging system such as a microscope platform. [0004] Various biological processes can rely on tight spatio-temporal coordination between cells across tissue-scale three-dimensional (3D) space. Therefore, there is a need for a volumetric imaging tool that can image at cellular resolution, provide 3D segmentation, and be fast enough to faithfully capture dynamic processing. In some cases, scanning methods are utilized to meet the needs of biovolumetric imaging: the optical signal i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T15/00G03H1/00G03H1/22H04N13/30
CPCG02B21/0076G02B21/0088G02B21/367G02B21/0032G06T7/557G06T9/00G06T15/08G06V10/141G06V10/60H04N19/597H04N13/254H04N13/111G02B21/0028
Inventor T.V.张S.马达恩D.B.霍兰德S.E.弗雷泽
Owner UNIV OF SOUTHERN CALIFORNIA
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