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Compound bactericide containing phenazine-1-formamide and ethylicin and application of compound bactericide
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A technology for compounding bactericide and acetalicin, applied in the directions of bactericide, application, biocide, etc., can solve problems such as drug resistance of germs, and achieve the effect of improving service life and being difficult to drug resistance.
Active Publication Date: 2018-12-28
HUNAN AGRICULTURAL UNIV
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At present, the commonly used chemical pesticides mainly include procymidone, pyrimethanil, and diethofencarb, etc., but long-term and large-scale use may cause the risk of bacterial resistance
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Embodiment 1
[0014] Example 1 The synergistic effect of phenazine-1-carboxamide and ethyl allicin compound on controlling Botrytis cinerea
[0015] (1) Test materials
[0016] The test bacteria used in this experiment were Botrytis cinerea cinerea from the Biocontrol Laboratory of Plant Protection College of Hunan Agricultural University.
[0017] The medicaments used in this experiment were allicin (80%, Beijing Zhongnong Jiarui Co., Ltd.), phenazine-1-carboxamide (95%, Shanghai Qinba Chemical Co., Ltd.).
[0018] Test medium: PDA medium (200g potato, 20g glucose, 20g agar, natural pH, add water to 1L, sterilize at 121°C for 20min at 1 atmospheric pressure).
[0019] (2) Toxicity determination of phenazine-1-carboxamide and acetoallin single dose
[0020] Weigh phenazine-1-carboxamide and acetoallin in 50mL beakers respectively, then add 2mL acetone and an appropriate amount of Tween 80 to the two beakers, shake and stir until the original drug is completely dissolved, and finally add d...
[0050] The test bacteria used in this experiment were Botrytis cinerea cinerea from the Biocontrol Laboratory of Plant Protection College of Hunan Agricultural University.
[0051] The medicaments used in this test are phenazine-1-carboxamide (95%, Shanghai Qinba Chemical Co., Ltd.) and 80% acetoallicin EC (80%, Beijing Zhongnong Jiarui Co., Ltd.), rot mold (95% , Shanghai Qinba Chemical Co., Ltd.).
[0052] Test medium: PDA medium (200g potato, 20g glucose, 20g agar, natural pH, add water to 1L, sterilize at 121°C for 20min at 1 atmospheric pressure).
[0054] Take phenazine-1-carboxamide, acetoallin and their complexes to prepare concentrations of 0.210859mg / mL, 1.389683mg / mL, and 0.213349mg / mL, respectively, for later use. There are 3 treatments in this test, 1 drug control (0.80mg / mL), 1 blank contr...
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Abstract
The invention relates to the field of pesticide compounding, in particular to a compound bactericide containing phenazine-1-formamide and ethylicin and an application of the compound bactericide. Thetoxicity of phenazine-1-formamide and ethylicin single agents and a compound agent thereof is tested with a growth rate method, and meanwhile, the synergistic effect of the compound agent is evaluatedwith a co-toxicity coefficient method. Results show that phenazine-1-formamide and ethylicin have bacteriostatic activity for botrytis cinerea of lilium brownie and have the EC50 being 21.0859 mu g / mL and 138.9683 mu g / mL respectively. The phenazine-1-formamide and ethylicin are compounded, when the weight ratio of the phenazine-1-formamide to ethylicin is 1:1, the co-toxicity coefficient is 172larger than 120, and the synergistic effect is remarkable. In-vitro leaf control effect test results show that the phenazine-1-formamide and ethylicin have the control effect of 83.85% on the botrytiscinerea of lilium brownie when compounded in the ratio being 1:1 and have potential of further development of novel pesticides.
Description
technical field [0001] The invention belongs to the field of pesticide compounding, and in particular relates to a compound fungicide containing phenazine-1-carboxamide and acetoallin, and also relates to the application of the fungicide in preventing and controlling gray mold of Lilium longya. Background technique [0002] Lilium brownii var. viridulum belongs to the genusLilium of the family Liliaceae and is a perennial herb. The bulbs of Longya lily resemble dragon teeth, so it is called "Dragon tooth lily". Longya lily is rich in trace elements that are beneficial to the human body, and the content of soluble harmful heavy metals is low. It is a green health food that integrates food and medicine. Longya lily is often disturbed by many factors during the growth process, such as: temperature, humidity, fertilizer, light, diseases, insect pests and weeds, etc. Among them, the gray mold of A. important factor in yield and quality. The disease is mainly a fungal disease ...
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