Method for recovering amino acids in canned sardine processing fish soup waste liquid and application thereof
A technology of canned sardines and a recovery method, which is applied in the field of amino acid recovery and sardine canned processing fish soup waste liquid, can solve the problems of increasing the economic cost of sewage treatment, environmental pollution, etc., and achieves the advantages of improving economic value and utilization value and reducing waste of resources. Effect
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Embodiment 1
[0019] Recovery method of amino acid in sardine canned fish broth waste liquid:
[0020] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2 times the mass for degreasing, the extracted upper layer solution is recovered with n-hexane, and the extracted lower layer aqueous solution is collected for use;
[0021] (2) Add the lower aqueous solution part to the treated strong acid styrene type cation exchange resin, wash the column with distilled water at a flow rate of 60 drops / min, first wash away inorganic salts, heavy metals and other substances, and use it as the effluent of the column When the reaction with 2% ninhydrin is positive, stop washing, use 3% ammonia water to elute slowly, control the flow rate to 80 drops / min, and collect the eluate, when the eluate is mixed with 2% ninhydrin When the reaction is negative, stop the elution;
[0022] (3) Concentrating the eluent collected in step (2) by rotary evaporation to remove ammonia gas and part...
Embodiment 2
[0025] Recovery method of amino acid in sardine canned fish broth waste liquid:
[0026] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2.5 times the mass for degreasing, and n-hexane is recovered from the extracted upper layer solution, and the extracted lower layer aqueous solution is collected for use;
[0027] (2) Add the lower aqueous solution part to the treated strong acidic styrene type cation exchange resin, wash the column with distilled water at a flow rate of 55 drops / min, first wash away inorganic salts, heavy metals and other substances, and use it as the effluent of the column When the reaction with 2% ninhydrin is positive, stop washing, and use 3.5% ammonia water to elute slowly, control the flow rate to 75 drops / min, and collect the eluate, when the eluate is mixed with 2% ninhydrin When the reaction is negative, stop the elution;
[0028] (3) Concentrating the eluent collected in step (2) by rotary evaporation to remove ammoni...
Embodiment 3
[0031] Recovery method of amino acid in sardine canned fish broth waste liquid:
[0032] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2 times the mass for degreasing, the extracted upper layer solution is recovered with n-hexane, and the extracted lower layer aqueous solution is collected for use;
[0033] (2) Add the aqueous solution of the lower layer to the treated strong acidic styrene-type cation exchange resin, wash the column with distilled water at a flow rate of 65 drops / min, first wash away inorganic salts, heavy metals and other substances, and use it as the effluent of the column When the reaction with 2% ninhydrin is positive, stop washing, and use 3% ammonia water to elute slowly, control the flow rate to be 85 drops / min, and collect the eluate, when the eluate is mixed with 2% ninhydrin When the reaction is negative, stop the elution;
[0034] (3) Concentrating the eluent collected in step (2) by rotary evaporation to remove amm...
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