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Application of combination of adenosine and autophagy inhibitor in preparation of drugs for treating colon cancer

A technology of autophagy inhibitors and therapeutic drugs, which is applied in the field of drugs, can solve problems such as unsatisfactory effects, and achieve good clinical therapeutic effects

Inactive Publication Date: 2018-12-28
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For patients who cannot be treated by surgery or to prevent metastasis after surgery, chemotherapy can only be continued, but the effect is generally not ideal

Method used

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  • Application of combination of adenosine and autophagy inhibitor in preparation of drugs for treating colon cancer
  • Application of combination of adenosine and autophagy inhibitor in preparation of drugs for treating colon cancer
  • Application of combination of adenosine and autophagy inhibitor in preparation of drugs for treating colon cancer

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Experimental method

[0032] (1) Cell culture and reagents

[0033] Human colon cancer cells SW620 and SW480 were purchased from the Cell Bank of the Chinese Academy of Sciences. Cells were cultured in DMEM+10% FBS. CQ, 3-MA, Hoechst33258 and acridine orange AO were purchased from Sigma. Purchased from Invitrogen in 2000. The main antibodies ATG5, Beclin1, p-Akt, mTOR, p-mTOR, p-70S6K, p-EBP1, and p62 were purchased from CellSignaling Inc, Danvers, USA, LC3 was purchased from Abclonal, USA, and Actin was purchased from Comverse Biotech.

[0034] (2) Recovery and subculture of colon cancer cell lines

[0035] Cell recovery: Take SW620 cells and SW80 cells out of the -80°C ultra-low temperature refrigerator, put them in a 37°C water bath, wait until they are completely melted, transfer them to a 15ml centrifuge tube with 5ml of medium, and centrifuge at 1200rpm for 5min. Discard the supernatant, add 1ml of fresh DMEM medium containing 10% FBS, mix well, transfer ...

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Abstract

The invention relates to the technical field of medicines, in particular to an application of adenosine and an autophagy inhibitor in the preparation of drugs for treating the colon cancer. The application has the advantage that the application firstly finds that the adenosine induces autophagy by inhibiting a PI3K / Akt / mTOR signaling pathway in colon cancer cells; from the order of phenotype, adenosine-induced apoptosis precedes the autophagy. Lowering of autophagy-related gene expression can promote the proportion of the adenosine-induced apoptosis in colon cancer cells, so the adenosine combined with the autophagy inhibitor (like chloroquine or 3-methyladenine) can kill the colon cancer cells more completely and achieve better clinical treatment effects.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to the application of a bio-metabolism ATP derivative: adenosine and an autophagy inhibitor in the preparation of a drug for treating colon cancer. Background technique [0002] Colon cancer is a common malignant tumor of the digestive tract that occurs in the colon. Its incidence rate ranks third among gastrointestinal tumors. Every year, there are more than one million new cases and about half a million deaths worldwide. The main causes of the disease are high-fat diet and insufficient fiber intake. Chronic inflammation of the colon makes the incidence of bowel cancer higher than that of the general normal population. With colon polyps, the incidence of colon cancer is 5 times that of those without colon polyps. Familial multiple bowel polyps have a higher incidence of cancer. Genetic factors may also be involved in the pathogenesis of colon cancer. Although surgical resec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61K31/52A61K31/4706A61K31/7048A61K31/585A61P35/00
CPCA61K31/4706A61K31/52A61K31/585A61K31/7048A61K31/7076A61P35/00A61K2300/00
Inventor 杨冬琴余顺吉候代森张文丽吴梦梦汤子慧吴利俊
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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