Oligopeptide and application in fishery field

A technology of oligopeptides and uses, applied in the chemical field, can solve the problems of reduced sperm life span and different sperm of Dabry's sturgeon, and achieve the effect of improving the antifreeze ability

Inactive Publication Date: 2018-12-28
魏清滨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, diluent and antifreeze are usually used for frozen storage of Dabry's sperm. The diluent is 0.7% sodium chloride + 0.02% potassium chloride + 3.5% gluc

Method used

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  • Oligopeptide and application in fishery field
  • Oligopeptide and application in fishery field
  • Oligopeptide and application in fishery field

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Experimental program
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Embodiment Construction

[0022] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0023] 1. Experimental materials

[0024] Oligopeptides A to D were synthesized by Shanghai Moxi Biotechnology Co., Ltd., with a purity of not less than 95%. The sequences are shown in Table 1.

[0025] Table 1 Oligopeptide A~D sequence

[0026]

[0027] Sodium chloride, potassium chloride, glucose, and DMSO are all conventional analytical reagents. Deionized water is homemade.

[0028] 2. Experimental method

[0029] 1. Sperm collection and pretreatment

[0030] From April to May of 2018, artificial ripening and birthing of sexually mature Dabry's sturgeon males cultivated by the Fisheries Research Institute of Sichuan Academy of Agricultural Sciences were carried out. Use a clean dry towel to wipe off the water and sundries around the body surface and genital ope...

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Abstract

The invention discloses oligopeptide and an application in the fishery field. At present, diluents and anti-freezing agents are generally used during cryopreservation of acipenser dabryanus sperm, thediluents adopt 0.7% sodium chloride, 0.02% potassium chloride and 3.5% glucose, the anti-freezing agents adopt dimethyl sulfoxide and the like, but these anti-freezing agents have different toxic effects on acipenser dabryanus sperm, and the sperm life is shortened after preservation. Study results prove that oligopeptide A, B or C is significantly superior to the prior art as the anti-freezing agent and can significantly prolong the life of sperm recovered from cryopreservation. The oligopeptide A, B or C can be used as the anti-freezing agent to improve the anti-freezing capacity of the acipenser dabryanus sperm.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to an oligopeptide and its application in the field of fishery. Background technique [0002] Dabry's sturgeon is a relatively large freshwater resident fish, which is endemic to my country. The meat and eggs of Dabry's sturgeon are delicious and rich in nutrition, especially the fish eggs can be processed into caviar. Due to rapid changes in the ecological environment due to the construction of water conservancy and overfishing, the wild population resources of Dabry's sturgeon are decreasing day by day. [0003] Low-temperature freezing technology can not only protect the rare species of Dabry's sturgeon, improve its reproductive potential and make it possible to carry out off-site or heterochronous fertilization, but also open up a new way for the protection of Dabry's sturgeon germplasm resources and biodiversity protection. It is also of great significance to provide long-...

Claims

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Application Information

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IPC IPC(8): C07K7/08A01N1/02
CPCC07K7/08A01N1/0221
Inventor 魏清滨严俊刚
Owner 魏清滨
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