Comprehensive extraction method for total triterpenoids and polysaccharides in ganoderma lucidum germ bran

A comprehensive extraction and Ganoderma lucidum technology, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve the problem of less research on the secondary utilization of Ganoderma lucidum chaff and the destruction of the three-dimensional structure of polysaccharides , polysaccharide extraction rate is not high, to achieve the effect of improving product competitiveness, shortening extraction time, and high extraction rate

Inactive Publication Date: 2018-12-28
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medium includes acid, alkali, hot water, ethanol, etc., but under the condition of dilute acid and dilute alkali, the glycosidic bonds of polysaccharides are easily broken, and some polysaccharides are hydrolyzed, which reduces the extraction rate of polysaccharides, and at the same time leads to the destruction of the three-dimensional structure of polysaccharides and The biological activity changes, and the extract needs to be neutralized, the procedure is cumbersome
Therefore, relatively speaking, hot water extraction is the most commonly used extraction method for extracting polysaccharides. It is easy to operate, takes a long time and high energy consumption, and the extraction rate of polysaccharides is not high.
[0005] At present, there are relatively few studies on the secondary utilization of Ganoderma lucidum chaff, and there is no report on the comprehensive extraction of Ganoderma lucidum triterpenes and polysaccharides from Ganoderma lucidum chaff

Method used

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  • Comprehensive extraction method for total triterpenoids and polysaccharides in ganoderma lucidum germ bran
  • Comprehensive extraction method for total triterpenoids and polysaccharides in ganoderma lucidum germ bran
  • Comprehensive extraction method for total triterpenoids and polysaccharides in ganoderma lucidum germ bran

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Selection: select the ganoderma fungus bag after the mushroom is picked, and choose the ganoderma fungus bag with white mycelium and pollution-free ganoderma;

[0040] 2) Pulverization: remove the outer bag from the ganoderma lucidum bag in step 1, and pulverize the ganoderma lucidum chaff, and pass through a 20-mesh sieve;

[0041] 3) Ultrasonic extraction: add 75% ethanol with 20 times the weight of the bacterial chaff to the pulverized fungus chaff in step 2), and use an ultrasonic cell breaker to carry out ultrasonic extraction at normal temperature and pressure, the ultrasonic power is 520w, and the extraction time is 5min , the single radiation time is 6s / 5s, and the obtained materials are used for standby.

[0042] 4) Centrifugation: Centrifuge the material in step 3) at a rotating speed of 3000r / min for 5min to obtain filter residue and clear liquid.

[0043] 5) Concentration: Evaporate the clear liquid described in step 4) under reduced pressure, the vacuum...

Embodiment 2

[0054] 1) Selection: select the ganoderma fungus bag after the mushroom is picked, and choose the ganoderma fungus bag with white mycelium and pollution-free ganoderma;

[0055] 2) Pulverization: remove the outer bag from the ganoderma lucidum bag in step 1, and pulverize the ganoderma lucidum chaff, and pass through a 20-mesh sieve;

[0056] 3) Ultrasonic extraction: add 75% ethanol with 15 times the weight of the bacterial chaff to the pulverized fungus chaff described in step 2), and use an ultrasonic cell breaker to carry out ultrasonic extraction at normal temperature and pressure, the ultrasonic power is 585w, and the extraction time 3min, single irradiation time 6s / 5s, the obtained materials are used for later use.

[0057] 4) Centrifugation: Centrifuge the material in step 3) at a rotating speed of 3000r / min for 5min to obtain filter residue and clear liquid.

[0058] 5) Concentration: Evaporate the clear liquid described in step 4) under reduced pressure, the vacuum ...

Embodiment 3

[0069] 1. Effect of ethanol concentration on the extraction rate of total triterpenes from Ganoderma lucidum residue

[0070] Accurately weigh 5.00 g of Ganoderma lucidum residue, 3 parts in total, add 50 mL of ethanol solution respectively, the ethanol concentration is 65%, 75%, 85% and 95% respectively. The extraction power is 60%, the single irradiation time is 6.0s / 5.0s, and ultrasonic extraction is performed at room temperature for 20 minutes. Then cool to room temperature, shake well, filter with filter paper to obtain the extract, take the filtrate, and dilute to 100mL, the solution is to be tested.

[0071] Depend on figure 1 It can be seen that in the range of 65% to 95% ethanol concentration, the extraction rate of total triterpenoids from Ganoderma lucidum residues gradually increases to the highest point and then slowly decreases, and the extraction rate of total triterpenes from Ganoderma lucidum residues reaches the highest when the ethanol concentration is 75% ...

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Abstract

The invention discloses a comprehensive extraction method for total triterpenoids and polysaccharides in ganoderma lucidum germ bran. The comprehensive extraction method comprises pulverizing the ganoderma lucidum germ bran and then adding ethanol, adopting an ultrasonic cell crusher for ultrasonic extraction, performing centrifugation after the ultrasonic extraction, and obtaining filter residueand clear solution; concentrating the obtained clear solution and then performing extraction with chloroform, evaporating and concentrating a chloroform layer under reduced pressure to a paste shape,and recycling the chloroform; washing the paste with ethanol, drying the ethanol, and obtaining the total triterpenoids of the ganoderma lucidum germ bran; using water to digest the obtained filter residue, and performing filtration after the digestion, to obtain filtrate; after the filtrate is concentrated, adding ethanol for precipitation, taking precipitate and performing desalting and drying,and then performing smashing to obtain ganoderma lucidum germ bran polysaccharide.

Description

technical field [0001] The invention relates to a comprehensive extraction method of total triterpenes and polysaccharides in ganoderma lucidum chaff, belonging to the technical field of intensive processing of agricultural and sideline products. Background technique [0002] In recent years, the production technology of Ganoderma lucidum has become more and more mature, and indoor cultivation and factory cultivation have basically been realized. With the increase of bagged Ganoderma lucidum year by year, a large amount of Ganoderma lucidum chaff is discarded every year, which not only causes the pollution of the surrounding environment, but also causes a serious waste of resources. The most important pharmacologically active substances in Ganoderma lucidum are triterpenes and polysaccharides of Ganoderma lucidum, which not only exist in the fruiting body, but also in the mycelium of the chaff of Ganoderma lucidum. Ganoderma lucidum triterpenoids have liver protection, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/074
CPCA61K36/074C08B37/0003A61K2236/53A61K2236/51A61K2236/333
Inventor 凡军民曹正谢春芹彭蓉谢正林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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