Kit and detection method for selectively detecting concentrations of pb ions and ag ions
An ion concentration and selectivity technology, which is applied in measurement devices, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inability to selectively detect multiple heavy metal ions, unsuitable for on-site detection, and low accuracy, and achieves good results. Recognition ability, multi-channel parallel detection, high sensitivity
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Embodiment 1
[0053] Example 1 Pb 2+ Drawing of detection standard curve
[0054] Take 10 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10, and do the following:
[0055] Add 920 μL of reagent II to No. 1 EP tube, and then add 40 μL with a concentration of 100×10 -6 mol / L reagent V, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L of reagent I, so that the solution volume is 1mL, to obtain solution 1;
[0056] Add 910 μL of Reagent II to No. 2 EP tube, and then add 10 μL of 10 -6 mol / L reagent III, 40 μL concentration is 100×10 -6 mol / L reagent V, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;
[0057] Add 900 μL of Reagent II to No. 3 EP tube, and then add 20 μL of 10 -6 mol / L reagent III, 40 μL concentration is 100×10 -6 mol / L reagent V, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 ...
Embodiment 2
[0068] Example 2 Ag + Drawing of detection standard curve
[0069] Take 12 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, and do the following:
[0070] Add 920 μL of reagent II to No. 1 EP tube, and then add 40 μL with a concentration of 100×10 -6 mol / L reagent VII, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L of reagent I, so that the solution volume is 1mL, to obtain solution 1;
[0071] Add 915 μL of reagent II to No. 2 EP tube, and then add 5 μL with a concentration of 100×10 -6 mol / L reagent IV, 40 μL concentration is 100×10 -6 mol / L reagent VII, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;
[0072] Add 910 μL of Reagent II to No. 3 EP tube, and then add 10 μL with a concentration of 100×10 -6 mol / L reagent IV, 40 μL concentration is 100×10 -6 mol / L reagent VII, mix well and incubate at 25°C ...
Embodiment 3
[0086] Carry out Pb under the condition of embodiment 1 2+ Concentration measurement, verification detection method of the present invention detects Pb 2+ ability to concentrate.
[0087] Take 3 EP tubes, labeled 1, 2 and 3, and do the following:
[0088] Add 840 μL of Reagent II to No. 1 EP tube, and then add 80 μL of 10 -6 mol / L reagent III, 40 μL concentration is 100×10 -6 mol / L reagent V, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 The reagent I of mol / L, make the solution volume be 1mL, obtain solution 1;
[0089] Add 720 μL of Reagent II to No. 2 EP tube, and then add 200 μL of 10 -6 mol / L reagent III, 40 μL concentration is 100×10 -6 mol / L reagent V, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;
[0090] Add 420 μL of Reagent II to No. 3 EP tube, and then add 500 μL of 10 -6 mol / L reagent III, 40 μL concentrat...
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