Construction method of highland barley-endophytic fungus symbiont

A technology of endophytic fungi and construction methods, applied in botany equipment and methods, horticulture, grain cultivation, etc., can solve the problems that the successful construction of highland barley-endophytic fungal symbiont has not been reported, and achieve good insect resistance effect

Active Publication Date: 2019-01-04
LANZHOU UNIVERSITY
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Problems solved by technology

[0004] At present, foreign countries have artificially inoculated pastures such as ryegrass and tall fescue, and successfully cons

Method used

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  • Construction method of highland barley-endophytic fungus symbiont
  • Construction method of highland barley-endophytic fungus symbiont
  • Construction method of highland barley-endophytic fungus symbiont

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Embodiment 1

[0035] The construction method of highland barley-endophytic fungi symbiont is as follows:

[0036] (1) Selection and processing of plant materials: select mature highland barley seeds with uniform color and size, rinse them under running water for 30 minutes, then treat them with 75% alcohol for 4 minutes, rinse them with sterile water for 4 times, and disinfect them with 10% sodium hypochlorite for 4 minutes. Rinse with water for 4 times, put the seeds on sterile filter paper, and wait until the seeds are dry. Among them, the variety of highland barley seeds is Chaiqing No. 1, provided by Gansu Academy of Agricultural Sciences.

[0037] (2) Preparation of seed inoculation medium: To prepare water agar medium, add 35 g of agar to 1 L of water, sterilize at 121° C. for 20 minutes with high-pressure damp heat, and pour it into a plate for later use.

[0038] (3) Cultivation of sterile seedlings: take the seeds that have been sterilized in step (1), place them on water agar medi...

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Abstract

The invention provides a construction method of a highland barley-endophytic fungus symbiont, which includes the following steps: (1) sterilization of highland barley seeds; (2) cultivation of highland barley sterile seedlings; (4) inoculation with fungi: artificially inoculating the epicotyls of the highland barley sterile seedlings with fungi: forming a small mouth at the epicotyls of the highland barley sterile seedlings through vertical cutting, inserting cultured hyphae into the small mouth, performing cultivation in dark at a constant temperature of 23-27 DEG C for 7-10 days, and performing cultivation under the conditions of the constant temperature being 23-27 DEG C, the illumination intensity being 2000-3000lx, and the illumination time being 16h.d<-1> for 7-10 days to obtain highland barley seedlings; (5) transplantation: after washing the highland barley seedlings to remove the culture medium at the roots, performing transplanting into a medium for culture until the seeds are harvested; and (6) detection of endophytic fungi: after transplanting for a period of time, detecting the inoculation situation of endophytic fungi, removing seedlings that the inoculation is unsuccessful, and collecting the seeds after the remaining seedlings are matured. The highland barley-endophytic fungus symbiont is constructed by inoculating the epicotyls of the highland barley with neotyphodium, and a new germplasm resource of the highland barley is created.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, and in particular relates to a method for constructing a highland barley-endophytic fungus symbiont. Background technique [0002] The dual characteristics of grass-endophyte symbionts have become one of the hot research areas in the past 20 years. It has been recognized by the international academic community that endophytic fungi can improve grass stress resistance and promote host growth. The infection of Neotyphodium endophytic fungi can also promote growth and improve competitiveness by enhancing drought resistance, salt tolerance, heavy metal tolerance and nutrient absorption; it can also increase host resistance to insects, nematodes, livestock, The resistance of wild animals, pathogenic fungi, and viruses affects the succession of grassland plant communities and changes the structure of the food chain. [0003] At present, about 10% of temperate grasses in the world have e...

Claims

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Application Information

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IPC IPC(8): A01G22/20A01G18/00
CPCA01G18/00A01G22/20
Inventor 李春杰王正凤
Owner LANZHOU UNIVERSITY
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