Construction method of highland barley-endophytic fungus symbiont

Abstract

The invention provides a construction method of a highland barley-endophytic fungus symbiont, which includes the following steps: (1) sterilization of highland barley seeds; (2) cultivation of highland barley sterile seedlings; (4) inoculation with fungi: artificially inoculating the epicotyls of the highland barley sterile seedlings with fungi: forming a small mouth at the epicotyls of the highland barley sterile seedlings through vertical cutting, inserting cultured hyphae into the small mouth, performing cultivation in dark at a constant temperature of 23-27 DEG C for 7-10 days, and performing cultivation under the conditions of the constant temperature being 23-27 DEG C, the illumination intensity being 2000-3000lx, and the illumination time being 16h.d<-1> for 7-10 days to obtain highland barley seedlings; (5) transplantation: after washing the highland barley seedlings to remove the culture medium at the roots, performing transplanting into a medium for culture until the seeds are harvested; and (6) detection of endophytic fungi: after transplanting for a period of time, detecting the inoculation situation of endophytic fungi, removing seedlings that the inoculation is unsuccessful, and collecting the seeds after the remaining seedlings are matured. The highland barley-endophytic fungus symbiont is constructed by inoculating the epicotyls of the highland barley with neotyphodium, and a new germplasm resource of the highland barley is created.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, and in particular relates to a method for constructing a highland barley-endophytic fungus symbiont. Background technique [0002] The dual characteristics of grass-endophyte symbionts have become one of the hot research areas in the past 20 years. It has been recognized by the international academic community that endophytic fungi can improve grass stress resistance and promote host growth. The infection of Neotyphodium endophytic fungi can also promote growth and improve competitiveness by enhancing drought resistance, salt tolerance, heavy metal tolerance and nutrient absorption; it can also increase host resistance to insects, nematodes, livestock, The resistance of wild animals, pathogenic fungi, and viruses affects the succession of grassland plant communities and changes the structure of the food chain. [0003] At present, about 10% of temperate grasses in the world have e...

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Problems solved by technology

[0004] At present, foreign countries have artificially inoculated pastures such as ryegrass and tall fescue, and successfully constructed artificial symbionts, but there is no report on the successful construction of highland barley-endophytic fungal symbionts

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