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Method for repairing cu, pb, as polluted soil by inoculating bacillus subtilis with sunflower and castor

A technology for Bacillus subtilis and contaminated soil, which is applied in the field of biological environmental remediation, and can solve the problems that the remediation technology is difficult to meet expectations, intolerable, and slow in plant growth.

Inactive Publication Date: 2021-05-04
江西夏氏春秋环境股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using phytoremediation technology to remediate Cu, Pb, As contaminated soil has good results, but there are many problems in the process of mass production and use, such as slow plant growth, poor stress resistance, and inability to tolerate Cu, Pb, As exceeding the standard soil , need to adjust measures to local conditions, etc.
At present, it has been found that castor has strong stress resistance and can grow normally in soils with excessive Cu, Pb, and As, but its ability to adsorb these elements alone is limited, and the toxicity of Cu, Pb, and As can inhibit the growth of castor. Therefore, a single repair technique is difficult to achieve the desired effect

Method used

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  • Method for repairing cu, pb, as polluted soil by inoculating bacillus subtilis with sunflower and castor
  • Method for repairing cu, pb, as polluted soil by inoculating bacillus subtilis with sunflower and castor

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example 1

[0045] (4) Cultivation: After the above-mentioned coated castor seeds and sunflower seeds are dried in the shade, they are sown in the contaminated soil, and mixed planting is carried out. The sunflower and castor are distributed at intervals, the plant distance is 0.5~1.0m, and the planting ratio of the two is 1:1;

[0046] (5) Management: When the height of the seedlings is 10cm, thin the seedlings, pull out the thin and thin seedlings that grow too densely, and combine with intertillage and weeding once. Carry out the third intertillage and weeding. When the height of the seedlings is 30cm, combine the intertillage to cultivate the soil and fix the roots;

[0047] (6) Removal and follow-up treatment: After a period of time, remove all castor and sunflower, and perform pollution-free treatment on the removed plants to extract Cu, Pb and As elements.

[0048] Further, the composition of the LB liquid medium in the step (1) is: tryptone 10g / L, yeast powder 5g / L, NaCl 5g / L, and...

Embodiment 1

[0059] Embodiment 1 adopts and utilizes sunflower, castor-oil plant to inoculate Bacillus subtilis to repair Cu, Pb, the method for As polluted soil to test the effect of the present invention, concrete steps are as follows:

[0060] Bacillus subtilis is transferred to LB liquid medium, and the composition of this LB liquid medium is: tryptone 10g / L, yeast powder 5g / L, NaCl 5g / L, adjust pH to be 7 with NaOH, in (29± 1) After culturing on a shaking table at ℃ for 24 hours, centrifuge at 8000r / min for 10 minutes in a refrigerated centrifuge, discard the supernatant, wash the bacteria twice with sterilized phosphate buffer, and finally dissolve the bacteria in sterile water, that is, It is a spare bacterial suspension; select plump castor and sunflower seeds, soak them in 1% NaClO for 20 minutes to disinfect the surface, then rinse them with sterile water and coat them in a bacterial suspension with a concentration of 109 CFU / mL for 30 minutes , the measurement of the seed coatin...

Embodiment 2

[0070] In order to thoroughly understand the method of utilizing sunflower, castor to inoculate Bacillus subtilis to restore Cu, Pb, As polluted soil in the present invention and deal with Cu, Pb, As compound polluted soil situation, now adopt castor to inoculate subtilis and sunflower to Ganzhou Haofeng Cu, Pb, As complex pollution site conducted a one-year remediation test.

[0071] The original Ganzhou Haofeng Metallurgical Chemical Industry Co., Ltd. is a private enterprise. Due to its backward production technology and equipment, the discharge of major pollutants seriously exceeds the standard, which not only wastes resources, but also seriously affects the quality of the regional environment. At present, the factory has completely stopped production, all buildings (structures) in the factory area have been dismantled, all equipment, raw and auxiliary materials and available equipment have been relocated, and the land of the factory site is in a state of abandonment. Soil...

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Abstract

The method of using sunflower and castor to inoculate Bacillus subtilis to restore Cu, Pb, As contaminated soil involves the field of environmental restoration technology, including preparation of bacterial suspension, seed inoculation, soil pretreatment, cultivation, management, removal and subsequent treatment, etc. The step is to use inoculated castor and inoculated sunflower to gradually transfer Cu, Pb, and As to the plants during the growth process, and then remove the whole plant from the soil to finally achieve the removal of excess Cu, Pb, and As in the soil. The invention has good effect on repairing Cu, Pb and As polluted soil, and is easy to implement and popularize.

Description

technical field [0001] The invention relates to the technical field of biological environment remediation, in particular to a method for remediating Cu, Pb and As pollution by using hyperaccumulator plants to inoculate Bacillus subtilis. Background technique [0002] Industrial development has brought economic take-off, but also brought serious pollution. Due to the simple and extensive production methods in my country at this stage, a large number of complex and highly toxic pollutants such as heavy metals (Cu, Pb, As, etc.) enter the environment, forming serious compound pollution. These pollutions, especially heavy metal pollution, can pass through the food chain and cause harm to animals, plants and humans themselves, and residents living in this environment also have serious health risks. [0003] Cu, Pb, As and other pollution are common polluting elements in industrial production, but at present, the research on Cu, Pb, As contaminated soil remediation at home and ab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/00B09C1/02B09C1/10
CPCB09C1/00B09C1/02B09C1/10B09C1/105
Inventor 夏小凯孙燕王江帆张燕
Owner 江西夏氏春秋环境股份有限公司