Livestock and poultry feed additive, livestock and poultry feed or drug containing genistein

A livestock and poultry feed, genistein technology, applied in animal feed, animal husbandry, food processing, etc., can solve the problems of slow contraction of red muscle fibers, low ATPase activity, etc., and achieve the effect of improving exercise endurance and meat quality

Active Publication Date: 2019-01-11
重庆市生猪产业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aerobic metabolism muscle fibers contain more myoglobin and cytochrome, and the color is bright red, so they are also called red muscle fibers (ie MyHC type I). This type of muscle fiber contains more mitochondria, and the enzyme activity of aerobic metabolism is high. Can obtain more energy, however, due to the lower ATPase activity compared with muscle fiber contraction, the contraction characteristics of red muscle fibers are slow and long-lasting

Method used

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  • Livestock and poultry feed additive, livestock and poultry feed or drug containing genistein
  • Livestock and poultry feed additive, livestock and poultry feed or drug containing genistein
  • Livestock and poultry feed additive, livestock and poultry feed or drug containing genistein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] in vitro test

[0045] (1) Genistein (purity 98%, mass purity), dissolved in dimethyl sulfoxide (DMSO) to prepare a mother solution with a concentration of 1 mmol / L, stored at -20°C, and reserved for cells.

[0046] (2) C2C12 cells (from stem cell bank, Chinese Academy of Sciences), at 37°C, 5% CO 2 (volume percent) conditions in the cell culture incubator for culture. Dulbecco's modified Eagle's medium (DMEM for short, Gibco, USA) supplemented with 10% fetal bovine serum (volume percentage) was used to make growth medium (GM) to maintain cell proliferation.

[0047] (3) When the C2C12 cells reached 80% confluency in the differentiation experiment, they were replaced with differentiation medium (DM) containing 2% horse serum (Gibco, volume percentage) for differentiation culture. When the cell density was about 70%, it was digested and passaged, and evenly spread in a 12-well plate. When the cells reached 80% confluence, the genistein mother solution was diluted to a ...

Embodiment 2

[0064] in vivo experiment

[0065] (1) Genistein was diluted step by step at concentrations of 0 mg / kg (control group) and 50 mg / kg (experimental group) and mixed into crushed mouse feed (mouse maintenance diet, purchased from Chengdu Dashuo Experimental Animal Co., Ltd. ) and then extruded and dried in an oven at 60°C for later use.

[0066] (2) Select 24 healthy ICR female mice at the age of 8 weeks, and randomly divide them into control group and experimental group, with 12 mice in each group. The mice in the control group and experimental group are fed with 0mg / kg and 50mg / kg genistein feed respectively For 4 weeks, the endurance of the mice was evaluated by swimming exercise experiment (water depth 60cm, water temperature 30±2°C), and the color difference instrument (model CR-300, Minota, Japan) was used to measure the exhaustion time of the mouse swimming exercise and the color change of the mouse leg muscles and mouse leg muscle color difference detection.

[0067] Am...

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Abstract

The present invention belongs to the technical field of a heterocyclic compound with a two six-membered carbocyclic fused heterocyclic compound containing an oxygen atom as an only heterocyclic atom and particularly relates to a livestock and poultry feed additive or a livestock and poultry feed or drug. The livestock and poultry feed additive or livestock and poultry feed or drug contains genistein. The livestock and poultry feed additive or livestock and poultry feed or drug can improve the meat quality of the livestock and poultry and improve the exercise tolerance of the livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of heterocyclic compounds containing oxygen atom as the only heterocyclic atom condensed by two six-membered carbon rings, and specifically relates to a livestock and poultry feed additive containing genistein or a livestock and poultry feed or medicine. Background technique [0002] Taking pork as an example, my country is the largest pig breeding and pork consumption country in the world. As a very important economic animal, the genetic improvement of pigs is of great significance to the development of my country's meat industry and people's daily life ( "Research progress on the growth and development of pig skeletal muscle", Zhao Xiao et al., Life Sciences, 2011, Vol. 23, No. 1, pp. 36-44, published on January 31, 2011). However, in the past few decades, the long-term breeding aimed at improving the lean meat rate and growth efficiency has caused a serious decline in the meat quality of many livestock and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K50/75A23K50/10A23K20/121
CPCA23K20/121A23K50/10A23K50/30A23K50/75Y02P60/87
Inventor 甘麦邻朱砺张顺华谭娅郭芝忺
Owner 重庆市生猪产业技术研究院有限公司
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