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Livestock and poultry feed additive or livestock and poultry feed or medicine containing genistein

A technology of genistein, livestock and poultry, applied in medical preparations containing active ingredients, animal feed, animal husbandry, etc., to achieve the effect of improving exercise endurance

Active Publication Date: 2022-06-14
重庆市生猪产业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aerobic metabolism muscle fibers contain more myoglobin and cytochrome, and the color is bright red, so it is also called red muscle fiber (ie MyHC type I). There are more mitochondria in the red muscle fiber, the enzyme activity of aerobic metabolism is high, and more energy can be obtained. However, because the ATPase activity associated with muscle fiber contraction is relatively low, the contraction characteristic of red muscle fiber is slow and long-lasting

Method used

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  • Livestock and poultry feed additive or livestock and poultry feed or medicine containing genistein
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  • Livestock and poultry feed additive or livestock and poultry feed or medicine containing genistein

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Experimental program
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Effect test

Embodiment 1

[0044] in vitro test

[0045] (1) Genistein (purity 98%, mass purity), dissolved in dimethyl sulfoxide (DMSO) to prepare a mother solution with a concentration of 1 mmol / L, stored at -20°C, and reserved for cells.

[0046] (2) C2C12 cells (from stem cell bank, Chinese Academy of Sciences), at 37°C, 5% CO 2 (volume percent) conditions were placed in a cell culture incubator for cultivation. Cell proliferation was maintained by using Dulbecco's modified Eagle's medium (DMEM for short, Gibco, USA) supplemented with 10% fetal calf serum (volume percentage) to form a growth medium (GM).

[0047] (3) When the C2C12 cells reached 80% confluence in the differentiation experiment, they were replaced with differentiation medium (DM) containing 2% horse serum (Gibco, volume percentage) for differentiation culture. When the cell density was about 70%, it was digested and passaged, and evenly spread in a 12-well plate. When the cells reached 80% confluence, the genistein mother solution ...

Embodiment 2

[0064] in vivo experiment

[0065] (1) Genistein was diluted step by step with 0mg / kg (control group) and 50mg / kg (experimental group) respectively and mixed into pulverized mouse feed (mouse maintenance diet, purchased from Chengdu Dashuo Experimental Animal Co., Ltd. ) and then extruded and dried in a 60°C oven for later use.

[0066] (2) Select 24 8-week-old healthy ICR female mice and randomly divide them into control group and experimental group, 12 in each group. The mice in the control group and experimental group are fed with 0mg / kg and 50mg / kg genistein feed respectively For 4 weeks, use the swimming exercise test (water depth 60cm, water temperature 30±2°C) to evaluate the endurance of the mice, and use a colorimeter (model CR-300, Minota, Japan) to measure the exhaustion time of the swimming exercise of the mice and the color changes of the leg muscles of the mice and mouse leg muscle color difference detection.

[0067] Among them, the detection method of the mou...

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Abstract

The invention belongs to the technical field of heterocyclic compounds containing oxygen atoms as the only heterocyclic atoms condensed by two six-membered carbon rings, and specifically relates to a livestock and poultry feed additive or a livestock and poultry feed or medicine. The livestock and poultry feed additive or livestock and poultry feed or medicine contains genistein. The livestock and poultry feed additive or livestock and poultry feed or medicine can improve the meat quality of livestock and poultry and improve the exercise endurance of livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of heterocyclic compounds containing oxygen atom as the only heterocyclic atom condensed by two six-membered carbon rings, and specifically relates to a livestock and poultry feed additive containing genistein or a livestock and poultry feed or medicine. Background technique [0002] Taking pork as an example, my country is the largest pig breeding and pork consumption country in the world. As a very important economic animal, the genetic improvement of pigs is of great significance to the development of my country's meat industry and people's daily life ( "Research progress on the growth and development of pig skeletal muscle", Zhao Xiao et al., Life Sciences, 2011, Vol. 23, No. 1, pp. 36-44, published on January 31, 2011). However, in the past few decades, the long-term breeding aimed at improving the lean meat rate and growth efficiency has caused a serious decline in the meat quality of many livestock and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A23K50/30A23K50/75A23K50/10A23K20/121
CPCA23K20/121A23K50/10A23K50/30A23K50/75Y02P60/87
Inventor 甘麦邻朱砺张顺华谭娅郭芝忺
Owner 重庆市生猪产业技术研究院有限公司