Application of PPM-18 in inducing apoptosis of bladder cancer cells by activating intracellular reactive oxygen species

A PPM-18, 1.PPM-18 technology, applied in the field of biomedicine, can solve the problems of large trauma, recurrence, human injury and so on

Active Publication Date: 2019-01-11
深圳泓达嘉企业管理咨询中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methods based on surgery, supplemented by radiotherapy and chemotherapy will cause major damage to the human body, and there is a risk of recurrence after surgery, and there are problems such as large trauma, obvious side effects, and low patient tolerance.

Method used

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  • Application of PPM-18 in inducing apoptosis of bladder cancer cells by activating intracellular reactive oxygen species
  • Application of PPM-18 in inducing apoptosis of bladder cancer cells by activating intracellular reactive oxygen species
  • Application of PPM-18 in inducing apoptosis of bladder cancer cells by activating intracellular reactive oxygen species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, PPM-18 anti-bladder cancer activity experiment

[0037] PPM-18 anti-bladder cancer activity experiment mainly includes the following steps:

[0038] (1-1) In vitro culture of bladder cancer cells used in experiments;

[0039] (1-2) using different concentrations of PPM-18 or PPM-18 to treat the cells cultured in step (1-1) for different times;

[0040] (1-3) Counting the detection of bladder cancer cell viability, apoptosis and ROS indicators treated with different concentrations of PPM-18 or PPM-18 for different times;

[0041] (1-4) According to the results of step (1-3), draw a graph of the detection results of the anti-bladder cancer activity of PPM-18.

[0042] The specific operation is as follows:

[0043] (1-1) Bladder cancer cell lines EJ and T24 were made into a single cell suspension with culture medium containing 10% fetal bovine serum, and 10,000 cells per well were seeded into a 96-well plate with a volume of 100 μl per well, and cultured u...

Embodiment 2

[0064] Embodiment 2, PPM-18 cell safety experiment

[0065] The PPM-18 cell safety experiment mainly includes the following steps:

[0066] (2-1) culturing the normal human hepatocytes used in the experiment in vitro;

[0067] (2-2) Treating the cells cultured in step (2-1) with different concentrations of PPM-18;

[0068] (2-3) Statistically detect the viability and apoptosis index of cells treated with different concentrations of PPM-18;

[0069] (2-4) According to the result of step (2-3), draw a graph of the cell safety detection result of PPM-18.

[0070] The specific operation is as follows:

[0071] (2-1) Normal human hepatocytes were prepared into a single cell suspension with culture medium containing 10% fetal bovine serum, seeded into a 96-well plate with 10,000 cells per well, and the volume of each well was 100 μl, and cultured until the cell abundance reached 80 %-90%.

[0072] (2-2) To the cells cultured in step (2-1), add PPM-18 such as 1 μM, 5 μM, 10 μM, ...

Embodiment 3

[0079] Example 3, PPM-18 can inhibit the viability of bladder cancer cells and the formation of clones, but has no obvious inhibitory effect on the viability of normal cells

[0080] In this embodiment, the experiment of PPM-18 inhibiting the viability of bladder cancer cells mainly includes the following steps:

[0081] (3-1) Cell culture: culture bladder cancer cells and normal human liver cells used in experiments in vitro, and digest and subculture the seed plate after the cells grow to the logarithmic growth phase;

[0082] (3-2) 96-well plate: the bladder cancer cell lines EJ and T24 were made into a single cell suspension with 10% fetal calf serum culture medium, and seeded into a 96-well plate with 10,000 cells per well, with a volume of 100 μl per well , cultivated until the cell abundance reaches 80%-90% (generally cultivated for 24 hours);

[0083] (3-3) Dosing treatment: after 24 hours of culture, gently suck out the old culture medium, and treat the cultured cell...

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Abstract

The invention provides an application of PPM-18 in inducing apoptosis of bladder cancer cells by activating intracellular reactive oxygen species (ROS). The PPM-18 significantly inhibits the viabilityof bladder cancer cell; PPM-18 promote cell apoptosis by inducing ROS production in bladder cancer cells; and the PPM-18 significantly inhibits the growth of human bladder cancer subcutaneous tumorsand prolongs the survival cycle of mice with subcutaneous tumors. At the same time, PPM-18 has very lower toxicity to normal human hepatocyte. In addition, PPM-It can be synthesized chemically, with lower cost, and can be used as a new drug for treating bladder cancer.

Description

technical field [0001] The invention relates to a new application of PPM-18, specifically, the application of PPM-18 to induce apoptosis of bladder cancer cells by activating intracellular reactive oxygen species (ROS), and belongs to the technical field of biomedicine. Background technique [0002] According to relevant reports, bladder cancer is one of the most common malignant tumors in the urinary system, ranking ninth among malignant tumors. In recent years, the morbidity and mortality of bladder cancer have been increasing, and it has become one of the malignant tumors that seriously affect and threaten human health and survival. Bladder cancer is divided into two branches: superficial and invasive. 80% of the former will have local recurrence, but the metastasis rate is less than 10%, and 50% to 80% of the latter will be metastasized. [0003] In recent years, great progress has been made in the treatment of invasive bladder cancer. At present, most of the treatments...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/167A61P35/00
CPCA61K31/167A61P35/00
Inventor 红凌梁华庚卢慧嫒
Owner 深圳泓达嘉企业管理咨询中心
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