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A whitening reducing polypeptide with the functions of inhibiting melanin production and anti-ultraviolet damage and its application

A technology of inhibiting melanin and anti-ultraviolet rays, applied in the field of biomedicine, can solve the problems of unstable biocompatibility, inability to have tyrosinase inhibition ability and anti-oxidation ability at the same time, and achieve normal cell protection and anti-oxidative aging cell, good biocompatibility effect

Active Publication Date: 2021-08-20
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a whitening reducing polypeptide with the effect of inhibiting melanin production and anti-ultraviolet damage and its application, so as to solve the problem that the existing peptide sequence cannot simultaneously have tyrosinase inhibitory ability and anti-oxidation ability and biocompatibility. stability problem

Method used

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  • A whitening reducing polypeptide with the functions of inhibiting melanin production and anti-ultraviolet damage and its application
  • A whitening reducing polypeptide with the functions of inhibiting melanin production and anti-ultraviolet damage and its application
  • A whitening reducing polypeptide with the functions of inhibiting melanin production and anti-ultraviolet damage and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Inhibitory effect of different concentrations of whitening reduced polypeptide ECGYF on tyrosinase

[0040] Experimental steps:

[0041]Saturatedly dissolve L-tyrosine in PBS buffer, the content of L-tyrosine is about 0.4mg / ml, add the whitening reduced peptide ECGYF with the concentration of 0mM, 0.6mM, 1.2mM, 3.1mM, 6.2mM respectively, After incubating at 37°C for ten minutes, 2KU units of tyrosinase preheated at 37°C were added, and after reacting for 24 hours, the light absorption value at 475nm was measured respectively, and the OD475nm represented the content of melanin.

[0042] Among them, the whitening reduced polypeptide ECGYF experiment with a concentration of 0 mM was used as a control group, and the OD 475nm of the control group was measured. Among them, the whitening reduced polypeptide ECGYF experiments with concentrations of 0.6mM, 1.2mM, 3.1mM, and 6.2mM were used as the experimental group, and the OD 475nm of the experimental group was measured.

[0...

Embodiment 2

[0049] Inhibitory effect of whitening reducing peptides ECGYF, ECAYF, ECVYF, ECYF and FYGCE on tyrosinase

[0050] Saturatedly dissolve L-tyrosine in PBS buffer, the content of L-tyrosine is about 0.4mg / ml, add the whitening reduced peptides ECGYF, ECAYF, ECVYF, ECYF and FYGCE at a concentration of 10mM respectively, and incubate at 37°C Ten minutes later, 2KU units of tyrosinase preheated at 37°C were added, and the light absorption values ​​at 475nm were measured before and after 24 hours of reaction.

[0051] Blank group: except that no whitening reducing peptides ECGYF, ECAYF, ECVYF, ECYF and FYGCE were added, the same incubation method, reaction method and measurement method as above were used.

[0052] Experimental results: As shown in Table 2, it shows that when G is replaced by A, V or deleted in the whitening reduced polypeptide ECGYF, or the sequence of ECGYF is reversed to FYGCE, the inhibitory effect on tyrosinase is similar to that of the whitening reduced polypep...

Embodiment 3

[0056] Comparison of IC50 values ​​of tyrosinase inhibitors (glutathione, arbutin and whitening reducing polypeptide ECGYF) on tyrosinase

[0057] Experimental method: configure different concentrations of glutathione, arbutin and whitening reducing polypeptide ECGYF, dynamically measure the production curve of each melanin in a 96-well plate, and obtain different concentrations of glutathione, arbutin and whitening reducing polypeptide ECGYF In the presence of the maximum reaction rate of tyrosinase, the IC50 of the minimum concentration of glutathione, arbutin and whitening reduced polypeptide ECGYF required respectively is calculated when the activity of tyrosinase is 50%.

[0058] Experimental results: if image 3 Shown, curve (b) is the tyrosinase activity inhibition curve of glutathione, and the IC50=1.18mM of glutathione; Curve (c) is the tyrosinase activity inhibition curve of arbutin, The IC50 of arbutin=5.73mM; the curve (a) is the tyrosinase activity inhibition cur...

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Abstract

The present invention relates to a whitening reducing polypeptide with the functions of inhibiting melanin production and resisting ultraviolet damage, and the whitening reducing polypeptide is a polypeptide having the amino acid sequence described in any one of SEQ ID NO:1-SEQ ID NO:5. The present invention also provides an application of the above-mentioned whitening reduced polypeptide, wherein the whitening reduced polypeptide is applied in the field of cosmetics or oral liquids. The whitening reduced polypeptide provided by the invention has both tyrosinase inhibitory ability and antioxidative ability, and has stable biocompatibility.

Description

technical field [0001] The invention relates to the field of biomedicine, and more specifically relates to a whitening reducing polypeptide capable of inhibiting melanin production and resisting ultraviolet damage and its application. Background technique [0002] Melanin generally plays an important role in protecting the human body from ultraviolet radiation. Ultraviolet rays can activate the activity of tyrosinase. After oxidizing tyrosine or 3,4-dihydroxyphenylalanine, melanin is formed through a series of chemical reactions. After melanin is produced, it can reduce the free radicals produced by ultraviolet rays and reduce the toxic effect of free radicals on normal cells. Excessive melanin formation can darken the skin, and excessive local production can lead to chloasma and freckles, both of which are skin diseases. In addition, excessive deposition of melanin is also an important sign of fruit and vegetable corruption. [0003] Tyrosinase is a key enzyme in the proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K5/113A61K8/64A61Q19/02A61Q19/08A61Q19/00A61Q17/04
CPCA61K8/64A61K2800/782A61K2800/92A61Q17/04A61Q19/004A61Q19/02A61Q19/08C07K5/1021C07K7/06
Inventor 沈志伟张益
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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